Selective media for the isolation of Actinobacillus pleuropneumoniae from the pig

Selective media were developed and evaluated for isolation of A. pleuropneumoniae from pig tonsils. Samples were obtained from four pig herds with a clinical history of pleuropneumonia. For isolation of A. pleuropneumoniae 93 pig tonsils were collected at slaughter Each sample was streaked on to four different selective media (modified PPLO agar (mPPLO), Brain-Heart agar (BH), Columbia agar (CA), Miller-Hinton chocolate agar (MHCA)) containing different combinations of antibiotics, NAD and nystatin. The selectivity of nutritive media is conditioned by the content of antibiotics, as well as by the type of medium used. Mean isolation rate of A. pleuropneumoniae in the investigated herds, was 17.2%. The best results were obtained using PPLO2 agar, 20.4%. The other media supplemented with the mentioned antibiotics gave satisfactory results.