A method for measuring mitochondrial mass and activity

被引：91

作者：

Agnello, Maria ^{[1
]}

Morici, Giovanni ^{[1
]}

Rinaldi, Anna Maria ^{[1
]}

机构：

[1] Univ Palermo, Dipartimento Biol Cellulare & Sviluppo A Monroy, I-90128 Palermo, Italy

来源：

CYTOTECHNOLOGY | 2008年 / 56卷 / 03期

关键词：

CLSM; mitochondrial mass; mitochondrial activity; Mitotracker Green; Mitotracker Orange; sea urchin;

D O I：

10.1007/s10616-008-9143-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.

引用

页码：145 / 149

页数：5

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