Modelling the enzymatic activity of two lipases isoenzymes commonly used in the food industry

被引：2

作者：

Guerra, Nelson P. ^{[1
]}

Pernas, Maria ^{[1
]}

Pastrana, Lorenzo ^{[1
]}

Torrado, Ana ^{[1
]}

Miguez, Martin ^{[1
]}

Fucinos, Clara ^{[1
]}

Estevez, Natalia ^{[1
]}

Sobrosa, Cristina ^{[1
]}

Gonzalez, Roberto ^{[1
]}

Fucinos, Pablo ^{[1
]}

Luisa Rua, Maria ^{[1
]}

机构：

[1] Univ Vigo, Fac Ciencias Ourense, Dept Quim Analit & Alimentaria, Orense 32004, Spain

来源：

CYTA-JOURNAL OF FOOD | 2011年 / 9卷 / 04期

关键词：

lipases; food; modelling; triacetin; hexane; Michaelis-Menten model; logistic model; INTERFACIAL ACTIVATION; KINETICS; PURIFICATION; INSIGHTS; SORPTION;

D O I：

10.1080/19476337.2011.601818

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An in-depth analysis of the kinetics of two lipases isoenzymes (Lip1 and Lip2) in triacetin hydrolysis in absence and in presence of hexane was carried out. The addition of hexane led to an increase in enzymatic activities of both enzymes for all triacetin concentrations, and the kinetic data described a hyperbola which was consistent with the classical Michaelis-Menten model. Without hexane, the time-course of the triacetin hydrolysis by Lip1 and Lip2 did not follow a Michaelian behaviour. In this case, a first phase of low enzymatic activity (at triacetin concentrations from 0 to 250 mM) was followed by a rapid increase in velocity at triacetin concentrations >= 250 mM. The Michaelis-Menten model was unable to describe the first phase due to the linear (nonhyperbolic) relationship between the velocity and the triacetin concentration, meanwhile the logistic model provided a satisfactory description of the experimental data corresponding to the second phase of activity.

引用

页码：307 / 313

页数：7