The inoculum size triggers tryptamine or secologanin biosynthesis in a Catharanthus roseus cell culture

被引：23
作者：
Contin, A
van der Heijden, R
ten Hoopen, HJG
Verpoorte, R
机构：
[1] Leiden Amsterdam Ctr Drug Res, Div Pharmacognosy, Gorlaeus Labs, NL-2300 RA Leiden, Netherlands
[2] Delft Univ Technol, Kluyver Lab Biotechnol, NL-2628 BC Delft, Netherlands
来源：
PLANT SCIENCE | 1998年 / 139卷 / 02期
关键词：
alkaloids; Catharanthus roseus; inoculum size; secologanin; tryptamine;
D O I：
10.1016/S0168-9452(98)00188-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A Catharanthus roseus cell line was characterized for the accumulation of secologanin, tryptophan, tryptamine, strictosidine and ajmalicine, as well as for the activities of the enzymes geraniol 10-hydroxylase, tryptophan decarboxylase and strictosidine synthase. The effects of the inoculum size on these parameters were discussed. The increase of the inoculum size from 40-160 g of fresh weight per liter of medium favored the accumulation of secologanin and alkaloids. A coordinated regulation of geraniol 10-hydroxylase and tryptophan decarboxylase by inoculum size seems to contribute to the effects observed. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
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收藏
页码：205 / 211
页数：7
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