Comparative Study of Two Point-of-Care Enzyme-Linked Immunosorbent Assays for the Detection of Antibodies against Canine Parvovirus and Canine Distemper Virus

This study was conducted to compare two commercially available point-of-care enzyme-linked immunosorbent assay kits (TiterCHEK (R) and ImmunoComb (R)) for the detection of antibodies against CPV and CDV. Dogs were clinically healthy, and included 22 (21%) beagles and 83 (79%) Korean native bobtailed dogs admitted to an animal shelter. Of the 105 dogs, 89 (84.76%) were found positive for CPV protective antibody titers (PATs) by HI assay and 3 (2.85%) were identified as positive for CDV PATs by SN assay. The CPV PAT was significantly higher (Pearson correlation coefficient= 0.297, P= 0.002) in adult dogs (>= 1 year) than that in young dogs (<1 year). For an accurate identification of CPV and CDV PATs, although not statistically significant, the specificity (100% for both CPV and CDV) and positive predictive values (PPV, 100% for both CPV and CDV) of the TiterCHEK were higher than those of the ImmunoComb (specificities for CPV and CDV, 81.3% and 97.1%; PPV for CPV and CDV, 81.3% and 97.1%, respectively). The TiterCHEK had fewer false-positive results than the ImmunoComb. To the best of our knowledge, this is the first study that shows compare sensitivity and specificity between the TiterCHEK (R) and ImmunoComb (R) kits, using CPV HI and CDV SN as reference tests. (C) 2017 PVJ. All rights reserved