Adiponectin regulates glycogen metabolism at the human fetal-maternal interface

被引:11
作者
Duval, Fabien [1 ]
Dos Santos, Esther [1 ,2 ]
Maury, Benoit [1 ]
Serazin, Valerie [1 ,2 ]
Fathallah, Khadija [3 ]
Vialard, Francois [1 ,3 ]
Dieudonne, Marie-Noelle [1 ]
机构
[1] Univ Versailles St Quentin En Yvelines, Univ Paris Saclay, Unite Format & Rech Sci Sante Simone Veil, GIG EA 7404, Montigny Le Bretonneux, France
[2] Ctr Hosp Poissy St Germain, Serv Biol Med, Poissy, France
[3] Ctr Hosp Poissy St Germain, Dept Biol Reprod Cytogenet Gynecol & Obstet, Poissy, France
关键词
adiponectin; human endometrium; human placenta; histiotrophic nutrition; glycogen metabolism; ENDOMETRIAL STROMAL CELLS; FULL-LENGTH ADIPONECTIN; IN-VITRO MODEL; INFERTILE PATIENTS; RECEPTOR-GAMMA; KAPPA-B; EXPRESSION; PROTEIN; DIFFERENTIATION; TROPHOBLASTS;
D O I
10.1530/JME-18-0013
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Throughout the entire first trimester of pregnancy, fetal growth is sustained by endometrial secretions, i.e. histiotrophic nutrition. Endometrial stromal cells (EnSCs) accumulate and secrete a variety of nutritive molecules that are absorbed by trophoblastic cells and transmitted to the fetus. Glycogen appears to have a critical role in the early stages of fetal development, since infertile women have low endometrial glycogen levels. However, the molecular mechanisms underlying glycogen metabolism and trafficking at the fetal-maternal interface have not yet been characterized. Among the various factors acting at the fetal-maternal interface, we focused on adiponectin - an adipocyte-secreted cytokine involved in the control of carbohydrate and lipid homeostasis. Our results clearly demonstrated that adiponectin controls glycogen metabol ism in EnSCs by (i) increasing glucose transporter 1 expression, (ii) inhibiting glucose catabolism via a decrease in lactate and ATP productions, (iii) increasing glycogen synthesis, (iv) promoting glycogen accumulation via phosphoinositide-3 kinase activation and (v) enhancing glycogen secretion. Furthermore, our results revealed that adiponectin significantly limits glycogen endocytosis by human villous trophoblasts. Lastly, we demonstrated that once glycogen has been endocytosed into placental cells, it is degraded into glucose molecules in lysosomes. Taken as a whole, the present results demonstrate that adiponectin exerts a dual role at the fetal-maternal interface by promoting glycogen synthesis in the endometrium and conversely reducing trophoblastic glycogen uptake. We conclude that adiponectin may be involved in feeding the conceptus during the first trimester of pregnancy by controlling glycogen metabolism in both the uterus and the placenta.
引用
收藏
页码:139 / 152
页数:14
相关论文
共 55 条
[1]   Adiponectin Inhibits Insulin Function in Primary Trophoblasts by PPARα- Mediated Ceramide Synthesis [J].
Aye, Irving L. M. H. ;
Gao, Xiaoli ;
Weintraub, Susan T. ;
Jansson, Thomas ;
Powell, Theresa L. .
MOLECULAR ENDOCRINOLOGY, 2014, 28 (04) :512-524
[2]   Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells [J].
Benaitreau, Delphine ;
Dos Santos, Esther ;
Leneveu, Marie-Christine ;
De Mazancourt, Philippe ;
Pecquery, Rene ;
Dieudonne, Marie-Noelle .
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2010, 8
[3]   Effects of adiponectin on human trophoblast invasion [J].
Benaitreau, Delphine ;
Dos Santos, Esther ;
Leneveu, Marie-Christine ;
Alfaidy, Nadia ;
Feige, Jean-Jacques ;
de Mazancourt, Philippe ;
Pecquery, Rene ;
Dieudonne, Marie-Noelle .
JOURNAL OF ENDOCRINOLOGY, 2010, 207 (01) :45-53
[4]   Antiproliferative Effects of Adiponectin on Human Trophoblastic Cell Lines JEG-3 and BeWo [J].
Benaitreau, Delphine ;
Dieudonne, Marie-Noelle ;
Dos Santos, Esther ;
Leneveu, Marie-Christine ;
de Mazancourt, Philippe ;
Pecquery, Rene .
BIOLOGY OF REPRODUCTION, 2009, 80 (06) :1107-1114
[5]   Morphological changes and protein secretion induced by progesterone in the endometrium during the luteal phase in preparation for nidation [J].
Bergeron, C .
HUMAN REPRODUCTION, 2000, 15 :119-128
[6]  
Bohlouli S, 2013, INT J FERTIL STERIL, V7, P43
[7]   Adiponectin-induced antiangiogenesis and antitumor activity involve caspase-mediated endothelial cell apoptosis [J].
Bråkenhielm, E ;
Veitonmäki, N ;
Cao, RH ;
Kihara, S ;
Matsuzawa, YJ ;
Zhivotovsky, B ;
Funahashi, T ;
Cao, YH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (08) :2476-2481
[8]   Adiponectin as a growth inhibitor in prostate cancer cells [J].
Bub, JD ;
Miyazaki, T ;
Iwamoto, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 340 (04) :1158-1166
[9]   AMP-activated protein kinase phosphorylates and inactivates liver glycogen synthase [J].
Bultot, Laurent ;
Guigas, Bruno ;
Von Wilamowitz-Moellendorff, Alexander ;
Maisin, Liliane ;
Vertommen, Didier ;
Hussain, Nusrat ;
Beullens, Monique ;
Guinovart, Joan J. ;
Foretz, Marc ;
Viollet, Benoit ;
Sakamoto, Kei ;
Hue, Louis ;
Rider, Mark H. .
BIOCHEMICAL JOURNAL, 2012, 443 :193-203
[10]   Human early placental development: Potential roles of the endometrial glands [J].
Burton, G. J. ;
Jauniaux, E. ;
Charnock-Jones, D. S. .
PLACENTA, 2007, 28 :S64-S69