The use of plasma donor-derived, cell-free DNA to monitor acute rejection after kidney transplantation

被引：72

作者：

Gielis, Els M. ^{[1
]}

Ledeganck, Kristien J. ^{[1
]}

Dendooven, Amelie ^{[2
]}

Meysman, Pieter ^{[3
,4
]}

Beirnaert, Charlie ^{[3
,4
]}

Laukens, Kris ^{[3
,4
]}

De Schrijver, Joachim ^{[5
]}

Van Laecke, Steven ^{[6
]}

Van Biesen, Wim ^{[6
]}

Emonds, Marie-Paule ^{[7
]}

De Winter, Benedicte Y. ^{[1
]}

Bosmans, Jean-Louis ^{[1
,8
]}

Del Favero, Jurgen ^{[5
]}

Abramowicz, Daniel ^{[1
,8
]}

机构：

[1] Univ Antwerp, Lab Expt Med & Pediat, Antwerp, Belgium

[2] Antwerp Univ Hosp, Dept Pathol, Antwerp, Belgium

[3] Univ Antwerp, Antwerp Univ Hosp, Biomed Informat Res Network Antwerp Biomina, Antwerp, Belgium

[4] Univ Antwerp, Dept Math & Comp Sci, Adv Database Res & Modelling ADReM, Antwerp, Belgium

[5] Multiplicom NV, Part Agilent Technol, Niel, Belgium

[6] Ghent Univ Hosp, Dept Nephrol, Renal Div, Ghent, Belgium

[7] Belgian Red Cross Flanders, Histocompatibil & Immunogenet Lab, Mechelen, Belgium

[8] Antwerp Univ Hosp, Dept Nephrol & Hypertens, Antwerp, Belgium

来源：

NEPHROLOGY DIALYSIS TRANSPLANTATION | 2020年 / 35卷 / 04期

关键词：

acute kidney rejection; biomarker; donor-derived cell-free DNA; kidney transplantation; HEMODIALYSIS; CLEARANCE; BIOMARKER; ASSAY;

D O I：

10.1093/ndt/gfz091

中图分类号：

R3 [基础医学]; R4 [临床医学];

学科分类号：

1001 ; 1002 ; 100602 ;

摘要：

Background. After transplantation, cell-free deoxyribonucleic acid (DNA) derived from the donor organ (ddcfDNA) can be detected in the recipient's circulation. We aimed to investigate the role of plasma ddcfDNA as biomarker for acute kidney rejection. Methods. From 107 kidney transplant recipients, plasma samples were collected longitudinally after transplantation (Day 1 to 3 months) within a multicentre set-up. Cell-free DNA from the donor was quantified in plasma as a fraction of the total cell-free DNA by next generation sequencing using a targeted, multiplex polymerase chain reaction-based method for the analysis of single nucleotide polymorphisms. Results. Increases of the ddcfDNA% above a threshold value of 0.88% were significantly associated with the occurrence of episodes of acute rejection (P=0.017), acute tubular necrosis (P=0.011) and acute pyelonephritis (P=0.032). A receiver operating characteristic curve analysis revealed an equal area under the curve of the ddcfDNA% and serum creatinine of 0.64 for the diagnosis of acute rejection. Conclusions. Although increases in plasma ddcfDNA% are associated with graft injury, plasma ddcfDNA does not outperform the diagnostic capacity of the serum creatinine in the diagnosis of acute rejection.

引用

页码：714 / 721

页数：8

共 25 条

[21] A Rapid Noninvasive Assay for the Detection of Renal Transplant Injury [J].

Sigdel, Tara K. ;

Vitalone, Matthew J. ;

Tran, Tim Q. ;

Dai, Hong ;

Hsieh, Szu-chuan ;

Salvatierra, Oscar ;

Sarwal, Minnie M. .

TRANSPLANTATION, 2013, 96 (01) :97-101

[22] Banff '09 Meeting Report: Antibody Mediated Graft Deterioration and Implementation of Banff Working Groups [J].

Sis, B. ;

Mengel, M. ;

Haas, M. ;

Colvin, R. B. ;

Halloran, P. F. ;

Racusen, L. C. ;

Solez, K. ;

Baldwin, W. M., III ;

Bracamonte, E. R. ;

Broecker, V. ;

Cosio, F. ;

Demetris, A. J. ;

Drachenberg, C. ;

Einecke, G. ;

Gloor, J. ;

Glotz, D. ;

Kraus, E. ;

Legendre, C. ;

Liapis, H. ;

Mannon, R. B. ;

Nankivell, B. J. ;

Nickeleit, V. ;

Papadimitriou, J. C. ;

Randhawa, P. ;

Regele, H. ;

Renaudin, K. ;

Rodriguez, E. R. ;

Seron, D. ;

Seshan, S. ;

Suthanthiran, M. ;

Wasowska, B. A. ;

Zachary, A. ;

Zeevi, A. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2010, 10 (03) :464-471

[23] Universal noninvasive detection of solid organ transplant rejection [J].

Snyder, Thomas M. ;

Khush, Kiran K. ;

Valantine, Hannah A. ;

Quake, Stephen R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (15) :6229-6234

[24] Evolution and Clinical Pathologic Correlations of De Novo Donor-Specific HLA Antibody Post Kidney Transplant [J].

Wiebe, C. ;

Gibson, I. W. ;

Blydt-Hansen, T. D. ;

Karpinski, M. ;

Ho, J. ;

Storsley, L. J. ;

Goldberg, A. ;

Birk, P. E. ;

Rush, D. N. ;

Nickerson, P. W. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2012, 12 (05) :1157-1167

[25] High-Resolution Profiling of Fetal DNA Clearance from Maternal Plasma by Massively Parallel Sequencing [J].

Yu, Stephanie C. Y. ;

Lee, Shara W. Y. ;

Jiang, Peiyong ;

Leung, Tak Y. ;

Chan, K. C. Allen ;

Chiu, Rossa W. K. ;

Lo, Y. M. Dennis .

CLINICAL CHEMISTRY, 2013, 59 (08) :1228-1237

← 1 2 3 →