Development and characterization of SARS-CoV-2 variant-neutralizing monoclonal antibodies

被引：2

作者：

Qiu, Hongyu ^{[1
]}

Yuan, Xin-Yong ^{[1
]}

Cabral, Teresa ^{[1
]}

Manguiat, Kathy ^{[1
]}

Robinson, Alyssia ^{[1
]}

Wood, Heidi ^{[1
]}

Grant, Chris ^{[1
]}

McQueen, Peter ^{[1
]}

Westmacott, Garrett ^{[1
]}

Beniac, Daniel R. ^{[1
]}

Lin, Lisa ^{[1
]}

Carpenter, Michael ^{[1
]}

Kobasa, Darwyn ^{[1
]}

Grafenhan, Tom ^{[1
,2
]}

机构：

[1] Publ Hlth Agcy Canada, Natl Microbiol Lab, 1015 Arlington St, Winnipeg, MB R3E 3R2, Canada

[2] Univ Wurzburg, Med Fac, Core Unit Syst Med SysMed, Josef Schneider Str 2, D-97080 Wurzburg, Germany

来源：

ANTIVIRAL RESEARCH | 2021年 / 196卷

关键词：

SARS-CoV-2; Broad-spectrum neutralizing mAbs; Variant of concern (VOC); PRNT; MUTATIONS; SPIKE;

D O I：

10.1016/j.antiviral.2021.105206

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Vaccination and administration of monoclonal antibody cocktails are effective tools to control the progression of infectious diseases and to terminate pandemics such as COVID-19. However, the emergence of SARS-CoV-2 mutants with enhanced transmissibility and altered antigenicity requires broad-spectrum therapies. Here we developed a panel of SARS-CoV-2 specific mouse monoclonal antibodies (mAbs), and characterized them based on ELISA, Western immunoblot, isotyping, and virus neutralization. Six neutralizing mAbs that exhibited high affinity binding to SARS-CoV-2 spike protein were identified, and their amino acid sequences were determined by mass spectrometry. Functional assays confirmed that three mAbs, F461G11, F461G15, and F461G16 neutralized four variants of concern (VOC): B.1.1.7 (alpha), B.1.351 (beta), P.1 (gamma) and B.1.617.2 (delta) These mAbs are promising candidates for COVID-19 therapy, and understanding their interactions with virus spike protein should support further vaccine and antibody development.

引用

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