Protocol for Efficient Generation and Characterization of Adeno-Associated Viral Vectors

被引：43

作者：

Jungmann, Andreas ^{[1
,2
]}

Leuchs, Barbara ^{[3
]}

Rommelaere, Jean ^{[3
]}

Katus, Hugo A. ^{[1
,2
]}

Mueller, Oliver J. ^{[1
,2
,4
]}

机构：

[1] Univ Hosp Heidelberg, Internal Med 3, Heidelberg, Germany

[2] German Ctr Cardiovasc Res DZHK, Heidelberg, Germany

[3] German Canc Res Ctr, Heidelberg, Germany

[4] Univ Kiel, Dept Internal Med 3, Arnold Heller Str 3, D-24105 Kiel, Germany

来源：

HUMAN GENE THERAPY METHODS | 2017年 / 28卷 / 05期

关键词：

adeno-associated virus (AAV); viral vector; vector purification; titration; quality control; ADENO-ASSOCIATED VIRUS; LARGE-SCALE PRODUCTION; GENE-TRANSFER; TITER; ENCAPSIDATION; PURIFICATION; TYPE-2; TRANSFECTION; TRANSDUCTION; REPLICATION;

D O I：

10.1089/hgtb.2017.192

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Adeno-associated virus vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high adeno-associated virus titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct comparability of different serotypes. In this protocol we describe a step-by-step procedure that results in well-characterized vectors suitable for both in vitro approaches and preclinical studies.

引用

页码：235 / 246

页数：12

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