Genotoxicity of arsenic evaluated by Allium-root micronucleus assay

被引：40
作者：
Yi, Huilan [1 ]
Wu, Lihua [1 ]
Jiang, Lin [1 ]
机构：
[1] Shanxi Univ, Coll Life Sci & Technol, Taiyuan 030006, Peoples R China
来源：
SCIENCE OF THE TOTAL ENVIRONMENT | 2007年 / 383卷 / 1-3期
基金：
中国国家自然科学基金;
关键词：
Genotoxicity; arsenic; Allium cepa; micronuclei; mitotic activity;
D O I：
10.1016/j.scitotenv.2007.05.015
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Arsenic exposure is associated with various diseases and cancers. By using Allium-root micronucleus (MN) assay, possible genotoxicity of sodium arsenite (0.3-100 mg/l) and arsenic trioxide (0.05-50 mg/l) was evaluated in this study. Our results showed that arsenic compounds induced MN formation concentration-dependently. Exposure to 0.5-20 mg/l arsenic trioxide or to 1-100 mg/l sodium arsenite caused MN significantly in meristernatic cells and daughter cells of Allium roots. A time-course study revealed that MN increased significantly after a short term (1 h) exposure to 10 mg/l sodium arsenitc, demonstrating an effective rapid response. Arsenic compounds also caused mitotic delay and a concentration-dependent decrease in mitotic index. Results of the present study suggest that Allium-root MN assay is a simple, efficient and reproducible method for the genotoxicity monitoring of arsenic water contamination. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：232 / 236
页数：5
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