Sage transcript profiles in cultured human fetal fibroblasts, WI-38

被引：4
|
作者：
Shklyaev, S [1 ]
Namba, H [1 ]
Hara, T [1 ]
Ohtsuru, A [1 ]
Yamashita, S [1 ]
机构：
[1] Nagasaki Univ, Sch Med, Dept Nat Med, Atom Bomb Dis Inst, Nagasaki 8528523, Japan
来源：
DNA SEQUENCE | 2000年 / 11卷 / 3-4期
关键词：
SAGE; human fibroblasts; DNA sequencing; tags; 7SL RNA (7L7);
D O I：
10.3109/10425170009033244
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Serial analysis of gene expression (SAGE) allows us to analyze the profile of genes expressed in human lung fibroblast cell line WI-38. Manual sequencing of approximately 10,000 transcripts derived from WI-38 cells revealed 1025 genes expression among of which 431 tags matched GenBank entries, whereas 594 corresponded to novel previously undescribed transcripts. 7SL RNA (7L7) 5'-truncated pseudogene and the gene with no match (tag: TCCCTAGCT) were found the most abundant among all the analyzed genes (10.2 and 4.6% respectively). The expression pattern of the genes coding for cytoskeletal proteins, extracellular matrix components (ECM) and cytokines has been obtained. The results here demonstrate the unique gene expression pattern in human WI-38 fibroblast cell line and support a possibility of useful application of SAGE method for differential expression analysis.
引用
收藏
页码：281 / 286
页数：6
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