Expression, purification, crystallization and preliminary X-ray analysis of the human NORE1 SARAH domain

被引：1

作者：

Kim, Hye Jin ^{[1
,2
]}

Hwang, Eunha ^{[1
,3
]}

Han, Young-Hyun ^{[1
]}

Choi, Saehae ^{[1
]}

Lee, Woo Cheol ^{[1
]}

Kim, Hye-Yeon ^{[1
]}

Jeon, Young Ho ^{[4
]}

Cheong, Chaejoon ^{[1
,2
]}

Cheong, Hae-Kap ^{[1
]}

机构：

[1] Korea Basic Sci Inst, Div Magnet Resonance, Ochang 363883, Chungbuk, South Korea

[2] Korea Univ Sci & Technol, Dept Bioanalyt Sci, Taejon 305350, South Korea

[3] Korea Univ, Div Biotechnol, Coll Life Sci & Biotechnol, Seoul 136701, South Korea

[4] Korea Univ, Coll Pharm, Jochiwon 339700, Chungnam, South Korea

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2012年 / 68卷

关键词：

TUMOR-SUPPRESSOR; IDENTIFICATION; PROTEINS; BINDING; PATHWAY;

D O I：

10.1107/S1744309112021744

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

NORE1 is an important tumour suppressor in human cancers that interacts with the pro-apoptotic protein kinase MST1/2 through SARAH domains. The SARAH domain (residues 366-413) of human NORE1 was expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 2.7 angstrom resolution and belonged to space group P6(1)22, with unit-cell parameters a = b = 73.041, c = 66.092 angstrom, alpha = beta = 90, gamma = 120 degrees.

引用

页码：813 / 815

页数：3

共 17 条

[1] Role of the ras-association domain family 1 tumor suppressor gene in human cancers
Agathanggelou, A
Cooper, WN
Latif, F
[J]. CANCER RESEARCH, 2005, 65 (09) : 3497 - 3508
[2] Norel inhibits tumor cell growth independent of Ras or the MST1/2 kinases
Aoyama, Y
Avruch, J
Zhang, XF
[J]. ONCOGENE, 2004, 23 (19) : 3426 - 3433
[3] Rassf Family of Tumor Suppressor Polypeptides
Avruch, Joseph
Xavier, Ramnik
Bardeesy, Nabeel
Zhang, Xian-Feng
Praskova, Maria
Zhou, Dawang
Xia, Fan
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (17) : 11001 - 11005
[4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5] Increasing complexity of Ras signaling
Campbell, SL
Khosravi-Far, R
Rossman, KL
Clark, GJ
Der, CJ
[J]. ONCOGENE, 1998, 17 (11) : 1395 - 1413
[6] An introduction to stereochemical restraints
Evans, Philip R.
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2007, 63 : 58 - 61
[7] Cell signaling: Life or death decisions of Ras proteins
Feig, LA
Buchsbaum, RJ
[J]. CURRENT BIOLOGY, 2002, 12 (07) : R259 - R261
[8] GTP-Ras disrupts the intramolecular complex of C1 and RA domains of Nore1
Harjes, E
Harjes, S
Wohlgemuth, S
Muller, KH
Krieger, E
Herrmann, C
Bayer, P
[J]. STRUCTURE, 2006, 14 (05) : 881 - 888
[9] Structural insight into dimeric interaction of the SARAH domains from Mst1 and RASSF family proteins in the apoptosis pathway
Hwang, Eunha
Ryu, Kyoung-Seok
Paakkonen, Kimmo
Guntert, Peter
Cheong, Hae-Kap
Lim, Dae-Sik
Lee, Jie-Oh
Jeon, Young Ho
Cheong, Chaejoon
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (22) : 9236 - 9241
[10] Identification of a novel Ras-regulated proapoptotic pathway
Khokhlatchev, A
Rabizadeh, S
Xavier, R
Nedwidek, M
Chen, T
Zhang, XF
Seed, B
Avruch, J
[J]. CURRENT BIOLOGY, 2002, 12 (04) : 253 - 265

← 1 2 →