Light-regulated interaction of dmoesin with TRP and TRPL channels is required for maintenance of photoreceptors

被引:40
作者
Chorna-Ornan, I
Tzarfaty, V
Ankri-Eliahoo, GA
Joel-Almagor, T
Meyer, NE
Huber, A
Payre, F
Minke, B [1 ]
机构
[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Physiol, IL-91120 Jerusalem, Israel
[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Kuhne Minerva Ctr Studies Visual Transduct, IL-91120 Jerusalem, Israel
[3] Univ Karlsruhe, Dept Cell Biol & Neurobiol, Inst Zool, D-76131 Karlsruhe, Germany
[4] Univ Toulouse 3, Ctr Biol Dev, F-31062 Toulouse, France
关键词
D O I
10.1083/jcb.200503014
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Recent studies in Drosophila melanogaster retina indicate that absorption of light causes the translocation of signaling molecules and actin from the photoreceptor's signaling membrane to the cytosol, but the underlying mechanisms are not fully understood. As ezrin-radixin-moesin (ERM) proteins are known to regulate actin-membrane interactions in a signal-dependent manner, we analyzed the role of Dmoesin, the unique D. melanogaster ERM, in response to light. We report that the illumination of dark-raised flies triggers the dissociation of Dmoesin from the light-sensitive transient receptor R potential (TRP) and TRP-like channels, followed by the migration of Dmoesin from the membrane to the cytoplasm. Furthermore, we show that light-activated migration of Dmoesin results from the dephosphorylation of a conserved threonine in Dmoesin. The expression of a Dmoesin mutant form that impairs this phosphorylation inhibits Dmoesin movement and leads to light-induced retinal degeneration. Thus, our data strongly suggest that the light- and phosphorylation-dependent dynamic association of Dmoesin to membrane channels is involved in maintenance of the photoreceptor cells.
引用
收藏
页码:143 / 152
页数:10
相关论文
共 39 条
  • [21] IDENTIFICATION OF A PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE-BINDING DOMAIN IN THE N-TERMINAL REGION OF EZRIN
    NIGGLI, V
    ANDREOLI, C
    ROY, C
    MANGEAT, P
    [J]. FEBS LETTERS, 1995, 376 (03) : 172 - 176
  • [22] TRPC5 activation kinetics are modulated by the scaffolding protein ezrin/radixin/moesin-binding phosphoprotein-50 (EBP50)
    Obukhov, AG
    Nowycky, MC
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2004, 201 (02) : 227 - 235
  • [23] Pearn MT, 1996, J BIOL CHEM, V271, P4937
  • [24] GENETIC DISSECTION OF LIGHT-INDUCED CA2+ INFLUX INTO DROSOPHILA PHOTORECEPTORS
    PERETZ, A
    SANDLER, C
    KIRSCHFELD, K
    HARDIE, RC
    MINKE, B
    [J]. JOURNAL OF GENERAL PHYSIOLOGY, 1994, 104 (06) : 1057 - 1077
  • [25] THE LIGHT RESPONSE OF DROSOPHILA PHOTORECEPTORS IS ACCOMPANIED BY AN INCREASE IN CELLULAR CALCIUM - EFFECTS OF SPECIFIC MUTATIONS
    PERETZ, A
    SUSSTOBY, E
    ROMGLAS, A
    ARNON, A
    PAYNE, R
    MINKE, B
    [J]. NEURON, 1994, 12 (06) : 1257 - 1267
  • [26] IDENTIFICATION OF A DROSOPHILA GENE ENCODING A CALMODULIN-BINDING PROTEIN WITH HOMOLOGY TO THE TRP PHOTOTRANSDUCTION GENE
    PHILLIPS, AM
    BULL, A
    KELLY, LE
    [J]. NEURON, 1992, 8 (04) : 631 - 642
  • [27] Small is beautiful: what flies tell us about ERM protein function in development
    Polesello, C
    Payre, F
    [J]. TRENDS IN CELL BIOLOGY, 2004, 14 (06) : 294 - 302
  • [28] Dmoesin controls actin-based cell shape and polarity during Drosophila melanogaster oogenesis
    Polesello, C
    Delon, I
    Valenti, P
    Ferrer, P
    Payre, F
    [J]. NATURE CELL BIOLOGY, 2002, 4 (10) : 782 - 789
  • [29] POLLOCK JA, 1995, J NEUROSCI, V15, P3747
  • [30] DEVELOPMENT OF DROSOPHILA RETINA, A NEUROCRYSTALLINE LATTICE
    READY, DF
    HANSON, TE
    BENZER, S
    [J]. DEVELOPMENTAL BIOLOGY, 1976, 53 (02) : 217 - 240