Effect of americium-241 on luminous bacteria. Role of peroxides

被引：33
作者：
Alexandrova, M. [1 ]
Rozhko, T. [1 ]
Vydryakova, G. [2 ]
Kudryasheva, N. [1 ,2 ]
机构：
[1] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[2] RAS, Inst Biophys SB, Krasnoyarsk 660036, Russia
来源：
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY | 2011年 / 102卷 / 04期
关键词：
Luminous bacteria; Radiotoxicity; Am-241; Hormesis; Peroxide; BIOLUMINESCENCE; ENUMERATION; EXTRACTION; MECHANISM; SAMPLES;
D O I：
10.1016/j.jenvrad.2011.02.011
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The effect of americium-241 (Am-241) an alpha-emitting radionuclide of high specific activity, on luminous bacteria Photobacterium phosphoreum was studied. Traces of Am-241 in nutrient media (0.16 -6.67 kBq/L) suppressed the growth of bacteria, but enhanced luminescence intensity and quantum yield at room temperature. Lower temperature (4 degrees C) increased the time of bacterial luminescence and revealed a stage of bioluminescence inhibition after 150 h of bioluminescence registration start. The role of conditions of exposure the bacterial cells to the Am-241 is discussed. The effect of Am-241 on luminous bacteria was attributed to peroxide compounds generated in water solutions as secondary products of radioactive decay. Increase of peroxide concentration in Am-241 solutions was demonstrated; and the similarity of 241Am and hydrogen peroxide effects on bacterial luminescence was revealed. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha-emitting radionuclides in aquatic solutions. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：407 / 411
页数：5
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