Enhancing sensitivity of liquid chromatographic/ion-trap tandem mass spectrometric determination of estrogens by on-line pre-column derivatization

被引：30

作者：

Matejicek, David ^{[1
]}

Kuban, Vlastimil ^{[1
]}

机构：

[1] Mendel Univ Brno, Dept Chem & Biochem, CZ-61300 Brno, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1192卷 / 02期

关键词：

estrogens; derivatization; liquid chromatography; ion-trap MS/MS; water;

D O I：

10.1016/j.chroma.2008.03.061

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive liquid chromatographic/ion-trap mass spectrometric method has been developed for the simultaneous determination of estriol, alpha-, beta-estradiol, 17 alpha-ethynylestradiol and estrone The sub. stances were extracted from river water samples (typically 250 ml) and cleaned-up using two-stage solid-phase extraction (SPE). 12-(Difluoro-1,3,5-triazinyl)-benz[f]isoindolo-[1,2b][1,3]benzothiazolidine was applied as a derivatizing agent. The recovery for each compounds ranged from 75 to 88% and the repeatability represented as RSDs ranged from 5.6 to 8.4%. Limits of detection (LOD, 3 x S/N) were 67-285 pg/l. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：248 / 253

页数：6

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