Hypoxia-induced secretion of macrophage migration-inhibitory factor from MCF-7 breast cancer cells is regulated in a hypoxia-inducible factor-independent manner

被引：31
作者：
Larsen, Mona [2 ]
Tazzyman, Simon [3 ]
Lund, Eva L. [2 ]
Junker, Nanna [2 ]
Lewis, Claire E. [3 ]
Kristjansen, Paul E. G. [2 ]
Murdoch, Craig [1 ]
机构：
[1] Univ Sheffield, Sch Clin Dent, Dept Oral & Maxillofacial Surg, Sheffield, S Yorkshire, England
[2] Univ Copenhagen, Inst Mol Pathol, Expt Oncol Lab, Copenhagen, Denmark
[3] Univ Sheffield, Sch Med, Div Genom Med, Acad Unit Pathol,Tumor Targeting Grp, Sheffield, S Yorkshire, England
来源：
CANCER LETTERS | 2008年 / 265卷 / 02期
基金：
英国生物技术与生命科学研究理事会;
关键词：
macrophage migration-inhibitory factor; hypoxia; hypoxia-inducible factor; breast cancer;
D O I：
10.1016/j.canlet.2008.02.012
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The cytokine MIF is over-expressed in tumors and is associated with tumor proliferation, angiogenesis and metastasis. Hypoxia, a hallmark feature of tumors, increases MIF expression from tumor cells. We examined the role of hypoxia-inducible transcription factors on MIF secretion from MCF-7 breast carcinoma cells. Secretion of MIF was induced by hypoxia after 24 h but up-regulation of MIF mRNA was minimal. Inhibition of HIF-1 alpha, HIF-2 alpha, NF-kappa B and C/EBP beta using siRNA had no effect on hypoxia-induced MIF secretion. However, inhibition of transcription and translation significantly decreased MIF production, suggesting that hypoxia-induced secretion of MIF in MCF-7 cells is via an alternative pathway. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：239 / 249
页数：11
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