Protective effects of Chlorella-derived peptide on UVB-induced production of MMP-1 and degradation of procollagen genes in human skin fibroblasts

被引：68

作者：

Chen, Chiu-Lan ^{[1
]}

Liou, Shu-Fen ^{[1
]}

Chen, Su-Jong ^{[1
]}

Shih, Mei-Fen ^{[1
]}

机构：

[1] Chia Nan Univ Pharm & Sci, Dept Pharm, Tainan, Taiwan

来源：

REGULATORY TOXICOLOGY AND PHARMACOLOGY | 2011年 / 60卷 / 01期

关键词：

UVB; MMP-1; CYR61; Procollagen; TbRII; c-fos; c-jun; MCP-1; Skin fibroblasts; Chlorella; MONOCYTE CHEMOATTRACTANT PROTEIN-1; GROWTH-FACTOR-BETA; UNICELLULAR GREEN-ALGAE; MATRIX METALLOPROTEINASE-1; KAPPA-B; CCN FAMILY; EXPRESSION; CELL; INFLAMMATION; COLLAGEN;

D O I：

10.1016/j.yrtph.2011.03.001

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

UV exposure is known to induce premature aging, which is mediated by matrix metalloproteinase-1 (MMP-1) activity. MMP-1 mRNA expression is up-regulated by elevated cysteine-rich 61 (CYR61) and monocyte chemoattractant protein-1 (MCP-1) via action of transcription factor AP-1. Collagen is degraded by MMP-1 activity but synthesized by transforming growth factor-beta (TGF-beta) signal. Chlorella has been shown to inhibit UVB-induced MMP-1 level, however its regulatory molecular mechanisms have not been studied. In this study, Chlorella derived peptide (CDP) was added to skin fibroblasts after UVB irradiation and the expression of MMP-1, CYR61, procollagen, c-fos, c-jun, and TGF-beta receptor (TbRII) mRNA and MCP-1 production were investigated. CDP (10 or 5 mg/ml) diminished UVB-induced MMP-1 and CYR61 mRNA expression and MCP-1 production, whereas. UVB-suppressed procollagen and TbRII mRNA was restored by CDP treatment. UVB-induced c-fos and c-jun expressions were also inhibited by the COP treatment. Taken together, CDP inhibits UVB-induced MMP-1 expression in skin fibroblasts by suppressing expression of AP-1 and CYR61 and MCP-1 production. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：112 / 119

页数：8

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