The quaternary state of polymerized human hemoglobin regulates oxygenation of breast cancer solid tumors: A theoretical and experimental study

被引:29
作者
Belcher, Donald A. [1 ]
Ju, Julia A. [2 ]
Baek, Jin Hyen [3 ]
Yalamanoglu, Ayla [3 ]
Buehler, Paul W. [3 ]
Gilkes, Daniele M. [2 ,4 ]
Palmer, Andre F. [1 ]
机构
[1] Ohio State Univ, William G Lowrie Dept Chem & Biomol Engn, Columbus, OH 43210 USA
[2] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD USA
[3] US FDA, Div Blood Components & Devices, Lab Biochem & Vasc Biol, CBER, Silver Spring, MD USA
[4] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21205 USA
来源
PLOS ONE | 2018年 / 13卷 / 02期
基金
美国国家卫生研究院;
关键词
PEG-CONJUGATED HEMOGLOBIN; CELL-FREE HEMOGLOBIN; BOVINE HEMOGLOBINS; TISSUE OXYGENATION; NITRIC-OXIDE; BLOOD-FLOW; BIOPHYSICAL PROPERTIES; ORTHOPEDIC-SURGERY; SOLUTION HBOC-201; 9L GLIOSARCOMA;
D O I
10.1371/journal.pone.0191275
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A major constraint in the treatment of cancer is inadequate oxygenation of the tumor mass, which can reduce chemotherapeutic efficacy. We hypothesize that polymerized human hemoglobin (PolyhHb) can be transfused into the systemic circulation to increase solid tumor oxygenation, and improve chemotherapeutic outcomes. By locking PolyhHb in the relaxed (R) quaternary state, oxygen (O-2) offloading at low O-2 tensions (< 20mm Hg) may be increased, while O-2 offloading at high O-2 tensions (> 20 mm Hg) is facilitated with tense (T) state PolyhHb. Therefore, R-state PolyhHb may deliver significantly more O-2 to hypoxic tissues. Biophysical parameters of T and R-state PolyhHb were used to populate a modified Krogh tissue cylinder model to assess O-2 transport in a tumor. In general, we found that increasing the volume of transfused PolyhHb decreased the apparent viscosity of blood in the arteriole. In addition, we found that PolyhHb transfusion decreased the wall shear stress at large arteriole diameters (> 20 mu m), but increased wall shear stress for small arteriole diameters (< 10 mu m). Therefore, transfusion of PolyhHb may lead to elevated O-2 delivery at low pO(2). In addition, transfusion of R-state PolyhHb may be more effective than T-state PolyhHb for O-2 delivery at similar transfusion volumes. Reduction in the apparent viscosity resulting from PolyhHb transfusion may result in significant changes in flow distributions throughout the tumor microcirculatory network. The difference in wall shear stress implies that PolyhHb may have a more significant effect in capillary beds through mechano-transduction. Periodic top-load transfusions of PolyhHb into mice bearing breast tumors confirmed the oxygenation potential of both PolyhHbs via reduced hypoxic volume, vascular density, tumor growth, and increased expression of hypoxia inducible genes. Tissue section analysis demonstrated primary PolyhHb clearance occurred in the liver and spleen indicating a minimal risk for renal damage.
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页数:31
相关论文
共 75 条
[11]   Tissue oxygenation after exchange transfusion with ultrahigh-molecular-weight tense- and relaxed-state polymerized bovine hemoglobins [J].
Cabrales, Pedro ;
Zhou, Yipin ;
Harris, David R. ;
Palmer, Andre F. .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2010, 298 (03) :H1062-H1071
[12]  
Camporesi EM., UNDERSEA HYPERB MED, V41, P253
[13]  
Castro C, 2014, MINERVA ANESTESIOL, V80, P537
[14]  
Chaturvedi P, 2012, J CLIN INVEST
[15]  
CHIANCONE E, 1968, J BIOL CHEM, V243, P1212
[16]   Hemoglobinuria-related acute kidney injury is driven by intrarenal oxidative reactions triggering a heme toxicity response [J].
Deuel, J. W. ;
Schaer, C. A. ;
Boretti, F. S. ;
Opitz, L. ;
Garcia-Rubio, I. ;
Baek, J. H. ;
Spahn, D. R. ;
Buehler, P. W. ;
Schaer, D. J. .
CELL DEATH & DISEASE, 2016, 7 :e2064-e2064
[17]   PERIVASCULAR OXYGEN-TENSIONS IN A TRANSPLANTABLE MAMMARY-TUMOR GROWING IN A DORSAL FLAP WINDOW CHAMBER [J].
DEWHIRST, MW ;
ONG, ET ;
KLITZMAN, B ;
SECOMB, TW ;
VINUYA, RZ ;
DODGE, R ;
BRIZEL, D ;
GROSS, JF .
RADIATION RESEARCH, 1992, 130 (02) :171-182
[18]  
Dewhirst MW, 1996, BRIT J CANCER, V74, pS247
[19]   Hypoxia decreased chemosensitivity of breast cancer cell line MCF-7 to paclitaxel through cyclin B1 [J].
Dong, X. L. ;
Xu, P. F. ;
Miao, C. ;
Fu, Z. Y. ;
Li, Q. P. ;
Tang, P. Y. ;
Wang, T. .
BIOMEDICINE & PHARMACOTHERAPY, 2012, 66 (01) :70-75
[20]   Blood Flow and Cell-Free Layer in Microvessels [J].
Fedosov, Dmitry A. ;
Caswell, Bruce ;
Popel, Aleksander S. ;
Karniadakis, George Em .
MICROCIRCULATION, 2010, 17 (08) :615-628