MicroRNA-21 is a new marker of circulating tumor cells in gastric cancer patients

被引：70

作者：

Zheng, Yuanyuan ^{[1
]}

Cui, Long ^{[1
]}

Sun, Weiliang ^{[2
,3
]}

Zhou, Hui ^{[1
]}

Yuan, Xianbin ^{[1
]}

Huo, Ming ^{[1
]}

Chen, Jie ^{[1
]}

Lou, Yanru ^{[3
,4
]}

Guo, Junming ^{[1
]}

机构：

[1] Ningbo Univ, Sch Med, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Univ, Ningbo Yinzhou Hosp, Ningbo 315211, Zhejiang, Peoples R China

[3] Ningbo Univ, Affiliated Hosp, Sch Med, Ningbo 315211, Zhejiang, Peoples R China

[4] Ningbo Univ, Ningbo Hosp 1, Ningbo 315211, Zhejiang, Peoples R China

来源：

CANCER BIOMARKERS | 2011年 / 10卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Gastric cancer; microRNA-21; gene diagnosis; circulating tumor cells; CLINICAL-SIGNIFICANCE; COLORECTAL-CANCER; CARCINOMA; EXPRESSION; BLOOD; INVASION; PROLIFERATION; ASSOCIATION; PROGNOSIS; DIAGNOSIS;

D O I：

10.3233/CBM-2011-0231

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The detection of circulating tumor cells (CTCs) has received great attention. MicroRNA-21 (miR-21) plays crucial roles in carcinogenesis and is considered as one of the most studied oncomiRNAs. We determined if miR-21 could be used a marker for the detection of CTCs in gastric cancer patients. Peripheral blood samples were collected from 53 preoperative patients with gastric cancer and 20 healthy volunteers. Real-time reverse transcription-polymerase chain reaction was used to detect the level of miR-21. Receiver operator characteristic curves (ROC) were constructed. Patients with gastric cancer display a significantly higher level of miR-21 in peripheral blood than those from controls. The miR-21 level was associated with the tumor node metastasis (TNM) stage, tumor size and tissue categories. The area under ROC curve was up to 0.853 +/- 0.086. This study highlights the potential of the detection of miR-21 in peripheral blood as a novel tool for monitoring CTCs in gastric cancer patients.

引用

页码：71 / 77

页数：7

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