Soluble tissue factor has unique angiogenic activities that selectively promote migration and differentiation but not proliferation of endothelial cells

被引：30

作者：

He, Yingbo ^{[1
]}

Chang, Guodong ^{[2
]}

Zhan, Shunli ^{[2
]}

Song, Xiaomin ^{[1
]}

Wang, Xiaofeng ^{[1
]}

Luo, Yongzhang ^{[1
]}

机构：

[1] Tsinghua Univ, Prot Chem Lab, Dept Biol Sci & Biotechnol, Beijing Key Lab Prot Therapeut, Beijing 100084, Peoples R China

[2] Protgen Ltd, Beijing 100085, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 370卷 / 03期

基金：

中国国家自然科学基金;

关键词：

tissue factor; angiogenesis; endothelial cells; migration; tubule formation; internalization;

D O I：

10.1016/j.bbrc.2008.03.133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The level of circulating tissue factor (TF) is up-regulated in human angiogenesis-related malignancies. However, whether circulating TF has angiogenic activities has not been determined. Soluble TF (sTF) is the main domain of circulating TF. Here, using cell migration, wound healing, and tubule formation assays, human recombinant sTF was found to significantly promote the migration and differentiation of endothelial cells. The stress fiber formation and rearrangement induced by sTF observed through immunofluorescence microscope may be responsible for the stimulatory migration effect of sTF. Nevertheless, sTF had no effects on endothelial cell proliferation. Interestingly, sTF can be internalized by endothelial cells, which implies a novel mechanism for sTF in angiogenesis. These results suggest that sTF has unique angiogenic activities and may serve as a potential therapeutic target to treat diseases associated with angiogenesis such as cancer and rheumatoid arthritis. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：489 / 494

页数：6

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