New Tools for Breaking Barriers to GPCR Expression in E. coli

被引:0
作者
Robinson, Anne [1 ]
机构
[1] Univ Delaware, Newark, DE 19716 USA
关键词
PROTEIN-COUPLED RECEPTORS; MEMBRANE-PROTEINS; PURIFICATION;
D O I
10.1016/j.jmb.2011.03.032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:597 / 598
页数:2
相关论文
共 12 条
[1]   Expression and purification of milligram levels of inactive G-protein coupled receptors in E-coli [J].
Bane, Steven E. ;
Velasquez, Javier E. ;
Robinson, Anne Skaja .
PROTEIN EXPRESSION AND PURIFICATION, 2007, 52 (02) :348-355
[2]   Over-expression, solubilization, and purification of G protein-coupled receptors for structural biology [J].
Chiu, Mark L. ;
Tsang, Cindy ;
Grihalde, Nelson ;
MacWilliams, Maria P. .
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, 2008, 11 (06) :439-462
[3]   Evolution of Three Human GPCRs for Higher Expression and Stability [J].
Dodevski, Igor ;
Plueckthun, Andreas .
JOURNAL OF MOLECULAR BIOLOGY, 2011, 408 (04) :599-615
[4]   Advances and challenges in membrane protein expression [J].
Link, A. James ;
Georgiou, George .
AICHE JOURNAL, 2007, 53 (04) :752-756
[5]  
Lundstrom Kenneth, 2006, Journal of Structural and Functional Genomics, V7, P77, DOI 10.1007/s10969-006-9011-2
[6]   Genetic selection system for improving recombinant membrane protein expression in E-coli [J].
Massey-Gendel, Elizabeth ;
Zhao, Anni ;
Boulting, Gabriella ;
Kim, Hye-Yeon ;
Balamotis, Michael A. ;
Seligman, Len M. ;
Nakamoto, Robert K. ;
Bowie, James U. .
PROTEIN SCIENCE, 2009, 18 (02) :372-383
[7]  
Robinson AS, 2011, PRODUCTION OF MEMBRANE PROTEINS: STRATEGIES FOR EXPRESSION AND ISOLATION, P1, DOI 10.1002/9783527634521
[8]   Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity [J].
Sarkar, Casim A. ;
Dodevski, Igor ;
Kenig, Manca ;
Dudli, Stefan ;
Mohr, Anja ;
Hermans, Emmanuel ;
Plueckthun, Andreas .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (39) :14808-14813
[9]   Simple Genetic Selection Protocol for Isolation of Overexpressed Genes That Enhance Accumulation of Membrane-Integrated Human G Protein-Coupled Receptors in Escherichia coli [J].
Skretas, Georgios ;
Georgiou, George .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (17) :5852-5859
[10]   Overexpression of mammalian integral membrane proteins for structural studies [J].
Tate, CG .
FEBS LETTERS, 2001, 504 (03) :94-98