Coronin 1C promotes triple-negative breast cancer invasiveness through regulation of MT1-MMP traffic and invadopodia function

被引:40
作者
Castagnino, Alessia [1 ]
Castro-Castro, Antonio [1 ]
Irondelle, Marie [1 ,2 ]
Guichard, Alan [1 ]
Lodillinsky, Catalina [1 ,3 ,4 ]
Fuhrmann, Laetitia [5 ]
Vacher, Sophie [6 ]
Aguera-Gonzalez, Sonia [1 ]
Zagryazhskaya-Masson, Anna [1 ]
Romao, Maryse [7 ]
El Kesrouani, Carole [5 ]
Noegel, Angelika A. [8 ]
Dubois, Thierry [9 ]
Raposo, Graca [7 ]
Bear, James E. [10 ,11 ]
Clemen, Christoph S. [8 ,12 ]
Vincent-Salomon, Anne [5 ]
Bieche, Ivan [6 ,13 ]
Chavrier, Philippe [1 ]
机构
[1] PSL Res Univ, CNRS, UMR144, Inst Curie,Membrane & Cytoskeleton Dynam Grp, 26 Rue Ulm, F-75005 Paris, France
[2] PSL Res Univ, Cell & Tissue Imaging Facil, Inst Curie, PICT,IBiSA, 26 Rue Ulm, F-75005 Paris, France
[3] Univ Buenos Aires, Fac Med, Inst Oncol AH Roffo, Area Invest, San Martin 5481,C1417DTB, Buenos Aires, DF, Argentina
[4] Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina
[5] PSL Res Univ, Inst Curie, Pathol Genet Immunol Dept, 26 Rue Ulm, F-75005 Paris, France
[6] PSL Res Univ, Inst Curie, Dept Genet, Pharmacogenom Unit, 26 Rue Ulm, F-75005 Paris, France
[7] PSL Res Univ, CNRS, Biogenesis & Funct Lysosome Related Organelles Gr, Inst Curie,UMR144, 26 Rue Ulm, F-75005 Paris, France
[8] Univ Cologne, Med Fac, Inst Biochem 1, Ctr Biochem, Joseph Stelzmann Str 52, D-50931 Cologne, Germany
[9] PSL Res Univ, Inst Curie, Translat Res Dept, Breast Canc Biol Grp, Paris, France
[10] Univ N Carolina, UNC Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27515 USA
[11] Univ N Carolina, Dept Cell Biol & Physiol, Chapel Hill, NC 27515 USA
[12] Ruhr Univ Bochum, Univ Hosp Bergmannsheil, Heimer Inst Muscle Res, Dept Neurol, Burkle de la Camp Pl 1, D-44789 Bochum, Germany
[13] Paris Descartes Univ, Sorbonne Paris Cite, Fac Pharmaceut & Biol Sci, EA7331, 4 Ave Observ, F-75006 Paris, France
关键词
ACTIN-BINDING; ARP2/3; COMPLEX; MATRIX-METALLOPROTEINASE; MOLECULAR-MECHANISMS; CORTACTIN PROMOTES; EXOSOME SECRETION; INVASION; WASH; METASTASIS; EXPRESSION;
D O I
10.1038/s41388-018-0422-x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane type 1-matrix metalloproteinase (MT1-MMP), a membrane-tethered protease, is key for matrix breakdown during cancer invasion and metastasis. Assembly of branched actin networks by the Arp2/3 complex is required for MT1-MMP traffic and formation of matrix-degradative invadopodia. Contrasting with the well-established role of actin filament branching factor cortactin in invadopodia function during cancer cell invasion, the contribution of coronin-family debranching factors to invadopodia-based matrix remodeling is not known. Here, we investigated the contribution of coronin 1C to the invasive potential of breast cancer cells. We report that expression of coronin 1C is elevated in invasive human breast cancers, correlates positively with MT1-MMP expression in relation with increased metastatic risk and is a new independent prognostic factor in breast cancer. We provide evidence that, akin to cortactin, coronin 1C is required for invadopodia formation and matrix degradation by breast cancer cells lines and for 3D collagen invasion by multicellular spheroids. Using intravital imaging of orthotopic human breast tumor xenografts, we find that coronin 1C accumulates in structures forming in association with collagen fibrils in the tumor microenvironment. Moreover, we establish the role of coronin 1C in the regulation of positioning and trafficking of MT1-MMP-positive endolysosomes. These results identify coronin 1C as a novel player of the multi-faceted mechanism responsible for invadopodia formation, MT1-MMP surface exposure and invasiveness in breast cancer cells.
引用
收藏
页码:6425 / 6441
页数:17
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