A novel DNA-binding motif in MarA: The first structure for an AraC family transcriptional activator

被引：239

作者：

Rhee, S ^{[1
]}

Martin, RG ^{[1
]}

Rosner, JL ^{[1
]}

Davies, DR ^{[1
]}

机构：

[1] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 18期

关键词：

D O I：

10.1073/pnas.95.18.10413

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 Angstrom thereby bending the DNA by approximate to 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.

引用

页码：10413 / 10418

页数：6

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