Urchinlike MnO2 nanoparticles for the direct electrochemistry of hemoglobin with carbon ionic liquid electrode

被引:70
作者
Zhu, Zhihong [2 ,3 ]
Qu, Lining [1 ]
Niu, Qingjuan [1 ]
Zeng, Yan [2 ,3 ]
Sun, Wei [1 ]
Huang, Xintang [2 ,3 ]
机构
[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China
[2] Huazhong Normal Univ, Inst Nanosci, Wuhan 430079, Peoples R China
[3] Huazhong Normal Univ, Ctr Technol, Wuhan 430079, Peoples R China
基金
美国国家科学基金会;
关键词
Urchinlike MnO2 nanoparticle; Carbon ionic liquid electrode; Hemoglobin; Direct electrochemistry; ELECTROCATALYSIS; FILMS; PROTEIN; ACIDS;
D O I
10.1016/j.bios.2010.09.017
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this paper an urchinlike MnO2 nanoparticle was synthesized by hydrothermal method and applied to the protein electrochemistry for the first time. By using a carbon ionic liquid electrode (CILE) as the basal electrode, hemoglobin (Hb) was immobilized on the surface of CILE with chitosan (CTS) and MnO2 nanoparticle composite materials. Spectroscopic results indicated that Hb molecules retained its native structure in the composite film. A pair of well-defined redox peaks appeared on the cyclic voltammogram with the formal peak potential as -0.180 V (vs. SCE), which indicated that direct electron transfer of Hb was realized on the modified electrode. The result can be attributed to the specific characteristic of MnO2 nanoparticle and the advantages of CILE, which facilitated the electron transfer rate. The fabricated CTS-MnO2-Hb/CILE showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA). Under the optimal conditions the catalytic current was in linear to TCA concentration in the range from 0.5 to 16.0 mmol L-1 with the detection limit calculated as 0.167 mmol L-1 (3 sigma). The result indicated that urchinlike MnO2 nanoparticle had the potential application in the third generation electrochemical biosensors. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:2119 / 2124
页数:6
相关论文
共 37 条
[1]   Recent developments in faradaic bioelectrochemistry [J].
Armstrong, FA ;
Wilson, GS .
ELECTROCHIMICA ACTA, 2000, 45 (15-16) :2623-2645
[2]   Hydrogels of a conducting conjugated polymer as 3-D enzyme electrode [J].
Åsberg, P ;
Inganäs, O .
BIOSENSORS & BIOELECTRONICS, 2003, 19 (03) :199-207
[3]   The effect of hydrothermal conditions on the mesoporous structure of TiO2 nanotubes [J].
Bavykin, DV ;
Parmon, VN ;
Lapkin, AA ;
Walsh, FC .
JOURNAL OF MATERIALS CHEMISTRY, 2004, 14 (22) :3370-3377
[4]  
Bianco Pierre, 2002, J Biotechnol, V82, P393, DOI 10.1016/S1389-0352(01)00054-X
[5]  
Dekant W., 1985, NAUNYMSCHMIEDEBERG S, V24, P329
[6]   Electron-transfer reactivity and enzymatic activity of hemoglobin in a SP sephadex membrane [J].
Fan, CH ;
Wang, HY ;
Sun, S ;
Zhu, DX ;
Wagner, G ;
Li, GX .
ANALYTICAL CHEMISTRY, 2001, 73 (13) :2850-2854
[7]   N-methyl-N-alkylpyrrolidinium tetrafluoroborate salts:: ionic solvents and solid electrolytes [J].
Forsyth, S ;
Golding, J ;
MacFarlane, DR ;
Forsyth, M .
ELECTROCHIMICA ACTA, 2001, 46 (10-11) :1753-1757
[8]   The development of bioelectrochemistry [J].
Hill, HAO .
COORDINATION CHEMISTRY REVIEWS, 1996, 151 :115-123
[9]   Manganese dioxide graphite composite electrodes: Application to the electroanalysis of hydrogen peroxide, ascorbic acid and nitrite [J].
Langley, Cathryn E. ;
Sljukic, Biljana ;
Banks, Craig E. ;
Compton, Richard G. .
ANALYTICAL SCIENCES, 2007, 23 (02) :165-170
[10]   GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS [J].
LAVIRON, E .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) :19-28