Activation of apoptotic caspase-3 and nitric oxide synthase-2 in buccal mucosa with chronic alcohol ingestion

被引：0

作者：

Slomiany, BL ^{[1
]}

Piotrowski, J ^{[1
]}

Piotrowski, E ^{[1
]}

Slomiany, A ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Res Ctr, Newark, NJ 07103 USA

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1998年 / 45卷 / 06期

关键词：

buccal mucosa; alcoholism; caspase-3; NOS-2; apoptosis;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apoptosis, the process of programmed cell death, involves activation of caspase proteases cascade that remains under the regulatory control of nitric oxide. In this study, we investigated the activity of a key apoptotic protease, caspase-3, and the expression of nitric oxide synthase-2 (NOS-2) associated with buccal epithelial cells apoptosis induced by chronic ethanol diet. The assays revealed that a 7.9-fold enhancement in buccal epithelial cells apoptosis, observed in the alcohol diet group, was accompanied by a 37.6-fold increase in caspase-3 activity and a 10.1-fold increase in NOS-2. Furthermore, the expression of NOS-2 showed a positive correlation (r = 0.92) with the extent of changes induced in caspase-3 activity. These results implicate caspase-3 in the process of alcohol-induced epithelial cells apoptosis, and point towards participation of NOS-2 in the amplification of the cell death signaling cascade.

引用

页码：1199 / 1209

页数：11

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