Requirements for green fluorescent protein detection in transgenic zebrafish embryos

被引：52
作者：
Amsterdam, A
Lin, S
Moss, LG
Hopkins, N
机构：
[1] MIT,CTR CANC RES,CAMBRIDGE,MA 02139
[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[3] TUFTS UNIV,NEW ENGLAND MED CTR,DIV ENDOCRINOL,BOSTON,MA 02111
来源：
GENE | 1996年 / 173卷 / 01期
关键词：
germ line; epifluorescence; GFP; microinjection; elongation factor 1-alpha;
D O I：
10.1016/0378-1119(95)00719-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We have generated transgenic (Tg) lines of zebrafish in which the green fluorescent protein (GFP)-encoding gfp cDNA is driven by the Xenopus laevis ef1 alpha enhancer/promoter, Tg embryos from most of these lines show detectable fluorescence throughout their body. We have investigated the copy number of the Tg genes in fluorescent and nonfluorescent lines, in order to determine how this affects the production of detectable levels of GFP in the zebrafish embryo, Additionally, we have injected purified recombinant GFP into embryos to determine the intracellular GFP concentration required for detection, both when all of the cells in the embryo contain GFP and when only a few do.
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收藏
页码：99 / 103
页数：5
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