Verification of pain-related neuromodulation mechanisms of icariin in knee osteoarthritis

被引：23

作者：

Li, Xihai ^{[1
,2
]}

Xu, Yunteng ^{[2
,3
]}

Li, Hui ^{[2
,3
]}

Jia, Liangliang ^{[2
,3
]}

Wang, Jie ^{[4
,5
]}

Liang, Shengxiang ^{[6
,7
]}

Cai, Aoling ^{[4
,5
]}

Tan, Xue ^{[2
,3
]}

Wang, Lili ^{[2
,3
]}

Wang, Xiaoning ^{[1
,2
]}

Huang, Yanfeng ^{[2
,3
]}

Tao, Enxiang ^{[8
]}

Ye, Hongzhi ^{[2
,3
]}

Asakawa, Tetsuya ^{[8
,9
]}

机构：

[1] Fujian Univ Tradit Chinese Med, Coll Integrat Med, Fuzhou 350122, Peoples R China

[2] Fujian Key Lab Integrat Med Geriatr, Fuzhou 350122, Peoples R China

[3] Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350122, Fujian, Peoples R China

[4] Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China

[5] Innovat Acad Precis Measurement Sci, Wuhan 430071, Peoples R China

[6] Fujian Univ Tradit Chinese Med, Natl Local Joint Engn Res Ctr Rehabil Med Technol, Fuzhou 350122, Peoples R China

[7] Fujian Univ Tradit Chinese Med, Rehabil Ind Inst, Fuzhou 350122, Peoples R China

[8] Sun Yat Sen Univ, Affiliated Hosp 8, Dept Neurol, Shennanzhong Rd 3025, Shenzhen 518033, Guangdong, Peoples R China

[9] Fujian Univ Tradit Chinese Med, Res Base Tradit Chinese Med Syndrome, Fuzhou 350122, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2021年 / 144卷

关键词：

Icariin; Knee osteoarthritis; Neuropeptides; Functional magnetic resonance imaging; Virus retrograde tracing; pain-related genesc; GENE-RELATED PEPTIDE; SYNOVIAL-FLUID; SUBSTANCE-P; NEUROPATHIC PAIN; NEUROPEPTIDE-Y; RAT MODEL; EXPRESSION; JOINT; IMMUNOREACTIVITY; NEUROKININ;

D O I：

10.1016/j.biopha.2021.112259

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Knee osteoarthritis (KOA) is a common disease with no specific treatment. Icariin (ICA) is considered an agent for KOA. This study aimed to confirm the pain-related neuromodulation mechanisms of ICA on KOA. Three experiments were designed: (1) verifying the therapeutic effects of ICA in vivo and in vitro, (2) exploring the potential pain-related neuromodulation pathways involved in ICA treatment by functional magnetic resonance imaging (fMRI) and virus retrograde tracing (VRT) and (3) confirming the pain-related targets by tandem mass tag (TMT)-based quantitative proteomics and bioinformatic analyses. Experiment 1 verified the efficacy of ICA in OA animal and cell models. Experiment 2 found a series of brain regions associated with KOA reversed by ICA treatment, indicating that a pain-related hypothalamic-mediated neuromodulation pathway and an endocannabinoid (EC)-related pathway contribute to ICA mechanisms. Experiment 3 explored and confirmed four pain-related genes involved in KOA and ICA treatment. We confirmed the key role of pain-related neuromodulation mechanisms in ICA treatment associated with its analgesic effect. Our findings contribute to considering ICA as a novel therapy for KOA.

引用

页数：14

共 39 条

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