Characterization of Ambrette Seed Oil and Its Mode of Action in Bacteria

被引：17

作者：

Arokiyaraj, Selvaraj ^{[1
]}

Choi, Seong Ho ^{[2
]}

Lee, Yoonseok ^{[1
]}

Bharanidharan, Rajaraman ^{[3
]}

Hairul-Islam, Villianur Ibrahim ^{[4
]}

Vijayakumar, Badathala ^{[5
]}

Oh, Young Kyoon ^{[6
]}

Dinesh-Kumar, Vannam ^{[7
]}

Vincent, Savariar ^{[7
]}

Kim, Kyoung Hoon ^{[1
,8
]}

机构：

[1] Seoul Natl Univ, Inst Green Bio Sci & Technol, Pyeongchang Daero 232916, South Korea

[2] Chungbuk Natl Univ, Dept Anim Sci, Chunbuk 361763, South Korea

[3] Anna Univ, Vel Tech High Tech Dr Rangarajan Dr Sakunthala En, Dept Biotechnol, Chennai 600062, Tamil Nadu, India

[4] Pondicherry Ctr Biol Sci, Pondicherry 605005, India

[5] Anna Univ, Vel Tech High Tech Dr Rangarajan Dr Sakunthala En, Dept Chem, Chennai 600062, Tamil Nadu, India

[6] Rural Dev Adm, Natl Inst Anim Sci, Dept Anim Nutr & Physiol, Suwon 441706, South Korea

[7] Loyola Coll, Ctr Environm Res & Dev, Chennai 600034, Tamil Nadu, India

[8] Seoul Natl Univ, Grad Sch Int Agr Technol, Pyeongchang 232916, South Korea

来源：

MOLECULES | 2015年 / 20卷 / 01期

关键词：

ambrette seed oil; antibacterial activity; chemical composition; docking; DHFR; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL RESISTANCE; PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; OREGANO;

D O I：

10.3390/molecules20010384

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, chemical composition and the antibacterial mechanism of ambrette seed oil are investigated. Chemical composition of the oil was analysed by gas chromatography-mass spectrometry (GC-MS). Thirty-five compounds were identified and the major compounds were found to be farnesol acetate (51.45%) and ambrettolide (12.96%). The antibacterial activity was performed by well diffusion assay and the mechanisms were studied by measuring the alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and protein leakage assays. The antibacterial effect of the ambrette seed oil showed inhibitory effect against Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis. The LDH activity was high in all tested bacteria compared with control, whereas the ALP and protein concentrations were also increased in E. faecalis. Molecular docking revealed the ligands farnesol acetate and ambrettolide had satisfactory binding energy towards the beta lactamase TEM-72 and dihydrofolate reductase (DHFR) protein. Due to its better antibacterial properties, the ambrette seed oil could be used as a source of antibacterial agents.

引用

页码：384 / 395

页数：12

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