Purification and Characterization of a Novel Anti-Proliferative Lectin from Morus alba L. Leaves

被引：25

作者：

Deepa, Mundekkad ^{[1
]}

Priya, Sulochana ^{[1
]}

机构：

[1] VIT Univ, CBST, Vellore 632014, Tamil Nadu, India

来源：

PROTEIN AND PEPTIDE LETTERS | 2012年 / 19卷 / 08期

关键词：

Mulberry leaf lectin; immobilized metal affinity chromatography; convective interaction media; haemagglutination; antiproliferative; cytotoxicity; AFFINITY-CHROMATOGRAPHY; MITOGENIC ACTIVITY; BINDING LECTIN; APOPTOSIS; COMBINATION; AGGLUTININ; FAMILY; SERUM;

D O I：

10.2174/092986612801619516

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel anti-proliferative lectin was purified from Morus alba L. (Mulberry) leaves by a two step chromatographic procedure namely, immobilized metal ion affinity chromatography (IMAC) and convective interaction media (CIM) based anion exchange chromatography. The purified mulberry leaf lectin (MLL) was specific to galactose, galactosamine and N-acetyl galactosamine (GalNAc). MLL was homogenous with a molecular weight of similar to 56kDa in silver stained SDS-PAGE. The lectin showed RBC agglutination activity up to 40 degrees C and was independent of pH above pH 6. Haemagglutination activity of purified MLL was not dependent on any metal ions. However, with high concentration of trivalent metal ions, Fe3+ and Al3+ and the divalent metal ion Fe2+, a three fold increase in agglutination activity was observed. The purified MLL showed an anti-proliferative activity towards human breast cancer cells (MCF-7) and colon cancer cells (HCT-15) with a higher potency towards MCF-7 cells. This is the first report on the anti-proliferative activity of a GalNAc specific lectin from M. alba.

引用

页码：839 / 845

页数：7

共 47 条

[1]

Adenike K, 2004, J BIOCHEM MOL BIOL, V37, P229

[2]

ALLEY MC, 1988, CANCER RES, V48, P589

[3] Polyhydroxylated alkaloids isolated from mulberry trees (Morus alba L.) and silkworms (Bombyx mori L.) [J].