Stomatin-like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis

被引:15
作者
Deng, Huan [1 ,2 ]
Deng, Yongjian [3 ]
Liu, Feiye [4 ]
Chen, Jie [5 ]
Li, Zheng [6 ]
Zhao, Kelei [1 ]
Guan, Xiaoqian [2 ]
Liang, Weijiang [1 ]
机构
[1] Southern Med Univ, Nanfang Hosp, Dept Oncol, 1838 Northern Ave, Guangzhou 510515, Guangdong, Peoples R China
[2] Southern Med Univ, Affiliated Hosp 3, Dept Oncol, Guangzhou 510630, Guangdong, Peoples R China
[3] Southern Med Univ, Nanfang Hosp, Dept Pathol, Guangzhou 510515, Guangdong, Peoples R China
[4] Southern Med Univ, Tradit Chinese Med Integrated Hosp, Canc Ctr, Guangzhou 510315, Guangdong, Peoples R China
[5] Southern Med Univ, Nanfang Hosp, Dept Obstet & Gynecol, Guangzhou 510515, Guangdong, Peoples R China
[6] Sun Yat Sen Univ, Canc Ctr, Dept Obstet & Gynecol, Guangzhou 510060, Guangdong, Peoples R China
关键词
stomatin-like protein 2; cervical cancer; prognosis; apoptosis; SLP-2; RADIATION; IDENTIFICATION; HYSTERECTOMY; CHEMOTHERAPY; CLONING; MEMBER; TIME;
D O I
10.3892/ol.2017.7054
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Stomatin-like protein 2 (SLP-2) is overexpressed in numerous types of human cancer and previous studies revealed that SLP-2 may function in mitochondria. The purpose of the present study was to evaluate the expression of SLP-2 in cervical cancer and the association between SLP-2 expression and clinical features, in addition to investigating the role of SLP-2 in the apoptosis of cervical cancer cells. The expression profile of SLP-2 was determined by quantitative polymerase chain reaction, western blotting and immunohistochemical staining. The effect of SLP-2 on cell apoptosis induced by chemotherapeutics in cervical cancer cells was evaluated using Annexin V staining and terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL) assays. The results indicated that SLP-2 expression in cervical cancer was significantly upregulated at the mRNA and protein levels, compared with that in normal cervical tissues. Immunohistochemical analysis revealed significant correlation between SLP-2 protein expression and clinical characteristics, including the squamous cell carcinoma antigen (P=0.003), deep stromal invasion (P=0.021), lymphovascular space involvement (P=0.044) and pelvic lymph node metastasis (P<0.001), which served as independent prognostic factors for predicting the shortening of overall survival time in patients with early-stage cervical cancer. In addition, TUNEL and Annexin V binding assays revealed that silencing SLP-2 expression significantly enhanced the sensitivity of cervical cancer cells to apoptosis induced by chemotherapeutics. Taken together, the results of the present study suggest that SLP-2 may be a progressive gene in the development of cervical cancer. Overexpression of SLP-2 serves an important role in the apoptosis of human cervical cancer cells.
引用
收藏
页码:6355 / 6364
页数:10
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