Water-Soluble Phosphorescent Ruthenium Complex with a Fluorescent Coumarin Unit for Ratiometric Sensing of Oxygen Levels in Living Cells

被引：53
作者：
Hara, Daiki [1 ]
Komatsu, Hirokazu [1 ]
Son, Aoi [1 ]
Nishimoto, Sei-ichi [1 ]
Tanabe, Kazuhito [1 ]
机构：
[1] Kyoto Univ, Grad Sch Engn, Dept Energy & Hydrocarbon Chem, Nishikyo Ku, Kyoto 6158510, Japan
来源：
BIOCONJUGATE CHEMISTRY | 2015年 / 26卷 / 04期
关键词：
TRANSITION-METAL-COMPLEXES; TRIPLET EXCITED-STATES; IN-VIVO; VISIBLE-LIGHT; IMAGING AGENT; TUMOR HYPOXIA; PROBE; CONJUGATE; SENSORS; NANOPARTICLES;
D O I：
10.1021/acs.bioconjchem.5b00093
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Dual emission was applied to a molecular probe for the ratiometric sensing of oxygen concentration in a living system. We prepared ruthenium complexes possessing a coumarin unit (Ru-Cou), in which the (MLCT)-M-3 phosphorescence of the ruthenium complex was efficiently quenched by molecular oxygen, whereas the coumarin unit emitted constant fluorescence independent of the oxygen concentration. The oxygen status could be determined precisely from the ratio of phosphorescence to fluorescence. We achieved the molecular imaging of cellular oxygen levels using Ru-Cou possessing an alkyl chain, which provided appropriate lipophilicity to increase cellular uptake.
引用
收藏
页码：645 / 649
页数：5
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