Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling

被引：6
|
作者：
Akagi, Tatsuya [1 ]
Shimizu, Kouhei [1 ]
Takahama, Shoukichi [2 ]
Iwasaki, Takahiro [2 ]
Sakamaki, Kazuhiro [3 ]
Endo, Yaeta [1 ,2 ,4 ,5 ]
Sawasaki, Tatsuya [1 ,2 ,4 ,5 ]
机构：
[1] Ehime Univ, Cell Free Sci & Technol Res Ctr, Matsuyama, Ehime 7908577, Japan
[2] Ehime Univ, Venture Business Lab, Matsuyama, Ehime 7908577, Japan
[3] Kyoto Univ, Grad Sch Biostudies, Dept Anim Dev & Physiol, Kyoto 6068501, Japan
[4] Ehime Univ, Proteomed Res Ctr, Toon, Ehime 7910295, Japan
[5] RIKEN, Syst & Struct Biol Ctr, Yokohama, Kanagawa 2300045, Japan
来源：
FEBS LETTERS | 2011年 / 585卷 / 12期
关键词：
Caspase-8; Cell-free system; Interleukin-21; Cytokines; Apoptosis; PROTEIN-SYNTHESIS SYSTEM; T-CELL-ACTIVATION; KAPPA-B; APOPTOSIS; EXPRESSION; COMPLEX; DEATH; CLONING; GROWTH; CHAIN;
D O I：
10.1016/j.febslet.2011.04.031
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We screened a library of human single-transmembrane proteins (sTMPs), produced by a cell-free system, using a luminescent assay to identify those that can be cleaved by caspase-8 (CASP8). Of the 407 sTMPs screened, only the interleukin-21 receptor (IL21R), vezatin (VEZT), and carbonic anhydrase XIV were cleaved at Asp344, Asp655 and Asp53, respectively. We confirmed that IL21R and VEZT were also cleaved in apoptotic HeLa cells with the cleavage sites. Interestingly, IL21R was cleaved within 30 min after apoptosis induction. Furthermore the CASP8-cleaved form of IL21R did not induce phosphorylation at Tyr705 of STAT3. Our results suggest that the interleukin-21 signaling cascade is negatively regulated by CASP8. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：1835 / 1840
页数：6
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