The TPTE gene family:: cellular expression, subcellular localization and alternative splicing

被引：65
作者：
Tapparel, C
Reymond, A
Girardet, C
Guillou, L
Lyle, R
Lamon, C
Hutter, P
Antonarakis, SE
机构：
[1] Univ Geneva, Sch Med, Ctr Med, Div Med Genet, CH-1211 Geneva, Switzerland
[2] Inst Cent Hop Valaisans, Div Pathol, Sion, Switzerland
[3] CHU Vaudois, Unit Pathol, Lausanne, Switzerland
[4] Inst Cent Hop Valaisans, Unit Genet, Sion, Switzerland
来源：
GENE | 2003年 / 323卷
关键词：
TPTE; dual phophatase; transmembrane domains; Hsa21; splice variants; expression in spermatocytes II/prespermatids; human endogenous retrovirus;
D O I：
10.1016/j.gene.2003.09.038
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The human TPTE (Transmembrane Phosphatase with TEnsin homology) gene family encodes a PTEN-related tyrosine phosphatase with four potential transmembrane domains. Chromosomal mapping revealed multiple copies of the TPTE gene on chromosomes 13, 15, 21, 22 and Y. Human chromosomes 13 and 21 copies encode two functional proteins, TPIP (T-PTE and PTEN homologous Inositol lipid Phosphatase) and TPTE, respectively, whereas only one copy of the gene exists in the mouse genome. In the present study, we show that TPTE and TPIP proteins are expressed in secondary spermatocytes and/or prespermatids. In addition, we report the existence of several novel alternatively spliced isoforms of these two proteins with variable number of transmembrane domains. The latter has no influence on the subcellular localization of these different peptides as shown by co-immunofluorescence experiments. Finally, we identify another expressed TPTE copy, mapping to human chromosome 22, whose transcription appears to be under the control of the LTR of human endogenous retrovirus RTVL-H3. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：189 / 199
页数：11
相关论文
共 29 条
[1]   A testis-specific gene, TPTE, encodes a putative transmembrane tyrosine phosphatase and maps to the pericentromeric region of human chromosomes 21 and 13, and to chromosomes 15, 22, and Y [J].
Chen, HM ;
Rossier, C ;
Morris, MA ;
Scott, HS ;
Gos, A ;
Bairoch, A ;
Antonarakis, SE .
HUMAN GENETICS, 1999, 105 (05) :399-409
[2]   Mechanisms and consequences of activation of protein kinase B/Akt [J].
Downward, J .
CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (02) :262-267
[3]   Signal transduction - New exchange, new target [J].
Downward, J .
NATURE, 1998, 396 (6710) :416-417
[4]   SPLICING OF A HUMAN ENDOGENOUS RETROVIRUS TO A NOVEL PHOSPHOLIPASE-A2 RELATED GENE [J].
FEUCHTERMURTHY, AE ;
FREEMAN, JD ;
MAGER, DL .
NUCLEIC ACIDS RESEARCH, 1993, 21 (01) :135-143
[5]  
Furnari FB, 1998, CANCER RES, V58, P5002
[6]   Genomic structure of a copy of the human TPTE gene which encompasses 87 kh on the short arm of chromosome 21 [J].
Guipponi, H ;
Yaspo, ML ;
Riesselman, L ;
Chen, HM ;
De Sario, A ;
Roizès, S ;
Antonarakis, SE .
HUMAN GENETICS, 2000, 107 (02) :127-131
[7]   The murine orthologue of the golgi-localized TPTE protein provides clues to the evolutionary history of the human TPTE gene family [J].
Guipponi, M ;
Tapparel, C ;
Jousson, O ;
Sacmuffa, N ;
Mas, C ;
Rossier, C ;
Hutter, P ;
Meda, P ;
Lyle, R ;
Reymond, A ;
Antonarakis, SE .
HUMAN GENETICS, 2001, 109 (06) :569-575
[8]   MEMBERS OF THE RTVL-H FAMILY OF HUMAN ENDOGENOUS RETROVIRUS-LIKE ELEMENTS ARE EXPRESSED IN PLACENTA [J].
JOHANSEN, T ;
HOLM, T ;
BJORKLID, E .
GENE, 1989, 79 (02) :259-267
[9]   Family ties of gated pores:: evolution of the sensor module [J].
Kumánovics, A ;
Levin, G ;
Blount, P .
FASEB JOURNAL, 2002, 16 (12) :1623-1629
[10]   Crystal structure of the PTEN tumor suppressor: Implications for its phosphoinositide phosphatase activity and membrane association [J].
Lee, JO ;
Yang, HJ ;
Georgescu, MM ;
Di Cristofano, A ;
Maehama, T ;
Shi, YG ;
Dixon, JE ;
Pandolfi, P ;
Pavletich, NP .
CELL, 1999, 99 (03) :323-334
123