Mucilaginibacter xinganensis sp. nov., a phenanthrene-degrading bacterium isolated from wetland soil

An aerobic, Gram-stain negative, rod-shaped and non-motile strain, BJC16-A31(T), was isolated from the wetland soil sample taken from Daxing'anling, Heilongjiang, People's Republic of China. Strain BJC16-A31(T) was found to be oxidase- and catalase-positive, and produced light orange colonies on modified R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BJC16-A31(T) is closely related to Mucilaginibacter gotjawali SA3-7(T) with 96.54% sequence similarity and it formed a separate lineage in the genus Mucilaginibacter. Strain BJC16-A31(T) contained menaquinone-7 (MK-7) as the predominant isoprenoid quinine. Anteiso-C-15:0, C-16:0 and anteiso-C-15:0 were the major fatty acids. The major polar lipids were phosphatidylethanolamine, six unidentified polar lipid, two unidentified aminophospholipids and one unidentified aminolipid. The genome is composed of a circular 5,301,339bp chromosome with average G+C percentage of 42.25%. The Average Nucleotide Identity (ANI) between strain BJC16-A31(T) and M. gotjawali SA3-7(T) was 77.51%. Combined phenotypic, chemotaxonomic, phylogenetic and genomic characteristics support the conclusion that strain BJC16-A31(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter xinganensis sp. nov. is proposed. The type strain is BJC16-A31(T) (=CGMCC 1.12728(T)=NBRC 110384(T)).