Involvement of B-Raf in Ras-induced Raf-1 activation

被引：23

作者：

Mizutani, S ^{[1
]}

Inouye, K ^{[1
]}

Koide, H ^{[1
]}

Kaziro, Y ^{[1
]}

机构：

[1] Tokyo Inst Technol, Fac Biosci & Biotechnol, Minato Ku, Yokohama, Kanagawa 2268501, Japan

来源：

FEBS LETTERS | 2001年 / 507卷 / 03期

关键词：

Ras; Raf-1; B-Raf;

D O I：

10.1016/S0014-5793(01)02992-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism of Ras-induced Raf-1 activation is not full), understood. Previously, we identified a 400-kDa protein complex as a Ras-dependent Raf-1 activator. In this study, we identified B-Raf as a component of this complex. B-Raf was concentrated during the purification of the activator. linamunodepletion of B-Raf abolished the effect of the activator on Raf-1. Furthermore, B-Raf and Ras-activated Raf-I co-operatively, when co-transfected into human embryonic kidney 293 cells. On the other hand, Ras-dependent extracellular signal-regulated kinase/mitogen-activated protein kinase kinase stimulator (a complex of B-Raf and 14-3-3) failed to activate Raf-I in our cell-free system. These results suggest that B-Raf is an essential component of the Ras-dependent Raf-I activator. Oc 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：295 / 298

页数：4

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