Resveratrol inhibits ACHN cells via regulation of histone acetylation

Context: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. Objective: To explore the functional role of resveratrol in RCC. Materials and methods: Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 mu g/mL, for 12, 24 and 48 h of culture) or 0.1 mu M SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. Results: When the cells were treated with 15.625, 31.25 and 62.5 mu g/mL resveratrol, ACHN cells viability was 73.2 +/- 3.5%, 61.4 +/- 3.1%, 50.2 +/- 4.7% for 12 h, 62.7 +/- 4.5%, 52.4 +/- 5.5%, 40.2 +/- 3.8% for 24 h, and 60.8 +/- 3.7%, 39.4 +/- 5.1%, 37.6 +/- 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 +/- 0.9%, 7.4 +/- 0.3% and 5.8 +/- 0.6%, and cell cycle was arrested at G1 from 42.5 +/- 2.9% to 55.3 +/- 5.7%, 59.8 +/- 3.4%, 68.7 +/- 4.6%. MMP-2/-9 expression (p < 0.05) was inhibited by resveratrol. The protein levels of histone acetylation (p < 0.01) was increased by resveratrol. Discussion and conclusions: Our results suggest that these effects might be related to a high level of histone acetylation, and resveratrol can be considered as an alternative treatment for RCC.