Osteogenic activities of MC3T3-E1 cells on heparin-immobilized poly(caprolactone) membranes

被引：19

作者：

Gumusderelioglu, Menemse ^{[1
]}

Karakecili, Ayse ^{[2
]}

Demirtas, T. Tolga ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Chem Engn & Bioengn, TR-06800 Ankara, Turkey

[2] Ankara Univ, Dept Chem Engn, TR-06100 Ankara, Turkey

来源：

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS | 2011年 / 26卷 / 03期

关键词：

heparin; osteogenic activity; poly(caprolactone); bone tissue engineering; covalent immobilization; FIBROBLAST-GROWTH-FACTOR; BONE MORPHOGENETIC PROTEIN-2; HUMAN-ENDOTHELIAL-CELLS; SURFACE MODIFICATION; CHITOSAN/HEPARIN COMPLEX; PCL MEMBRANES; DIFFERENTIATION; HYDROGELS; DELIVERY; PROLIFERATION;

D O I：

10.1177/0883911511406329

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this study was to investigate the effects of heparin on the activity of osteoblast-like cells seeded on poly(caprolactone) (PCL) membranes. The membranes were prepared by solvent-casting technique in similar to 150 mu m thickness. Then they were treated with 1,6-hexanediamine solution and functionalized with covalently bound heparin. The morphology, proliferation, and differentiation of MC3T3-E1 preosteoblasts on these membranes were investigated in vitro. The heparin functionalized PCL membranes, compared to non-functionalized membranes, significantly stimulated osteoblast proliferation. The Scanning electron microscope images confirmed the stimulative effect of covalently bound heparin on the osteoblast-like cell proliferation. The alkaline phosphatase and osteocalcin levels for cells proliferated on heparin containing PCL membranes were higher than that of nonfunctionalized membranes.

引用

页码：257 / 269

页数：13

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