Clostridia in Premature Neonates' Gut: Incidence, Antibiotic Susceptibility, and Perinatal Determinants Influencing Colonization

被引:56
作者
Ferraris, Laurent [1 ]
Butel, Marie Jose [1 ]
Campeotto, Florence [1 ]
Vodovar, Michel [2 ]
Roze, Jean Christophe [3 ]
Aires, Julio [1 ]
机构
[1] Univ Paris 05, Fac Sci Pharmaceut & Biol, EA4065, Paris, France
[2] Inst Puericulture, Paris, France
[3] Univ Nantes, Dept Med Neonatale, Ctr Invest Clin, CHU Nantes,INSERM,CIC004, Nantes, France
关键词
PRETERM INFANTS; NECROTIZING ENTEROCOLITIS; BACTERIAL-COLONIZATION; MICROFLORA; MICROBIOTA; DIFFICILE; FLORA; RESISTANT; BOWEL; PCR;
D O I
10.1371/journal.pone.0030594
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Although premature neonates (PN) gut microbiota has been studied, data about gut clostridial colonization in PN are scarce. Few studies have reported clostridia colonization in PN whereas Bacteroides and bifidobacteria have been seldom isolated. Such aberrant gut microbiota has been suggested to be a risk factor for the development of intestinal infections. Besides, PN are often treated by broad spectrum antibiotics, but little is known about how antibiotics can influence clostridial colonization based on their susceptibility patterns. The aim of this study was to report the distribution of Clostridium species isolated in feces from PN and to determine their antimicrobial susceptibility patterns. Additionally, clostridial colonization perinatal determinants were analyzed. Results: Of the 76 PN followed until hospital discharge in three French neonatal intensive care units (NICUs), 79% were colonized by clostridia. Clostridium sp. colonization, with a high diversity of species, increased throughout the hospitalization. Antibiotic courses had no effect on the clostridial colonization incidence although strains were found susceptible (except C. difficile) to anti-anaerobe molecules tested. However, levels of colonization were decreased by either antenatal or neonatal (during more than 10 days) antibiotic courses (p = 0.006 and p = 0.001, respectively). Besides, incidence of colonization was depending on the NICU (p = 0.048). Conclusion: This study shows that clostridia are part of the PN gut microbiota. It provides for the first time information on the status of clostridia antimicrobial susceptibility in PN showing that strains were susceptible to most antibiotic molecules. Thus, the high prevalence of this genus is not linked to a high degree of resistance to antimicrobial agents or to the use of antibiotics in NICUs. The main perinatal determinant influencing PN clostridia colonization appears to be the NICU environment.
引用
收藏
页数:7
相关论文
共 51 条
[1]   An outbreak of necrotizing enterocolitis associated with a novel Clostridium species in a neonatal intensive care unit [J].
Alfa, MJ ;
Robson, D ;
Davi, M ;
Bernard, K ;
Van Caeseele, P ;
Harding, GKM .
CLINICAL INFECTIOUS DISEASES, 2002, 35 :S101-S105
[2]   FECAL BACTERIAL MICROFLORA OF NEWBORN-INFANTS DURING INTENSIVE-CARE MANAGEMENT AND TREATMENT WITH 5 ANTIBIOTIC REGIMENS [J].
BENNET, R ;
ERIKSSON, M ;
NORD, CE ;
ZETTERSTROM, R .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1986, 5 (05) :533-539
[3]   Mode of delivery affects the bacterial community in the newborn gut [J].
Biasucci, Giacomo ;
Rubini, Monica ;
Riboni, Sara ;
Morelli, Lorenzo ;
Bessi, Elena ;
Retetangos, Cristiana .
EARLY HUMAN DEVELOPMENT, 2010, 86 (01) :S13-S15
[4]   Enteral feeding induces diet-dependent mucosal dysfunction, bacterial proliferation, and necrotizing enterocolitis in preterm pigs on parenteral nutrition [J].
Bjornvad, Charlotte R. ;
Thymann, Thomas ;
Deutz, Nicolaas E. ;
Burrin, Douglas G. ;
Jensen, Soren K. ;
Jensen, Bent B. ;
Molbak, Lars ;
Boye, Mette ;
Larsson, Lars-Inge ;
Schmidt, Mette ;
Michaelsen, Kim F. ;
Sangild, Per T. .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2008, 295 (05) :G1092-G1103
[5]   DEVELOPMENT OF GUT COLONIZATION IN PRETERM NEONATES [J].
BLAKEY, JL ;
LUBITZ, L ;
BARNES, GL ;
BISHOP, RF ;
CAMPBELL, NT ;
GILLAM, GL .
JOURNAL OF MEDICAL MICROBIOLOGY, 1982, 15 (04) :519-529
[6]   ENTERIC COLONIZATION IN SPORADIC NEONATAL NECROTIZING ENTEROCOLITIS [J].
BLAKEY, JL ;
LUBITZ, L ;
CAMPBELL, NT ;
GILLAM, GL ;
BISHOP, RF ;
BARNES, GL .
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1985, 4 (04) :591-595
[7]   What have we learned about antimicrobial use and the risks for Clostridium difficile-associated diarrhoea? [J].
Blondeau, J. M. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 63 (02) :238-242
[8]   CLOSTRIDIUM-DIFFICILE IN PEDIATRIC INFECTIONS [J].
BROOK, I ;
AVERY, G ;
GLASGOW, A .
JOURNAL OF INFECTION, 1982, 4 (03) :253-257
[9]   CLOSTRIDIAL INFECTION IN CHILDREN [J].
BROOK, I .
JOURNAL OF MEDICAL MICROBIOLOGY, 1995, 42 (02) :78-82
[10]   The role of anaerobic bacteria in bacteremia [J].
Brook, Itzhak .
ANAEROBE, 2010, 16 (03) :183-189