Inhibition of TPA-induced cyclooxygenase-2 expression and skin inflammation in mice by wogonin, a plant flavone from Scutellaria radix

Wogonin (5.7-dihydroxy-8-methoxyflavone), isolated from Scutellaria radix. was previously reported to inhibit the expression and activity of the enzyme cyclooxygenase-2 in lipopolysaccharide (LPS)-stimulated cells of a mouse macrophage cell line, RAW 264.7. Here, in order to find in vivo effects, inhibition by wogonin of 12-O- tetradecanoylphorbol-13 -acetate (TPA)-induced cyclooxygenase-2 expression and anti-inflammatory activity in vivo were investigated. When applied topically to the dorsal skin of mice, wagonin at doses of 50-200 mug/site/treatment (total of five treatments in 3 days) inhibited cyclooxygenase-2 expression and prostaglandin E-2 production induced by multiple treatments with TPA. At 200 mug/site/treatment, wogonin caused a 55.3% reduction of prostaglandin E-2 production on the dorsal skin compared with an increased production in the TPA-treated control group. The same compound significantly inhibited mouse ear edema induced by TPA in both preventive (58.1% inhibition) as well as curative treatment (31.3% inhibition) schedules at 200 mug/ear/treatment. Inhibition of neutrophil infiltration was also observed. Therefore, wogonin may be beneficial for cyclooxygenase-2-related skin disorders. (C) 2001 Published by Elsevier Science BN.