The involvement of regulatory non-coding RNAs in sepsis: a systematic review

被引：78

作者：

Ho, Jeffery ^{[2
]}

Chan, Hung ^{[2
]}

Wong, Sunny H. ^{[1
,3
]}

Wang, Maggie H. T. ^{[4
]}

Yu, Jun ^{[1
,3
]}

Xiao, Zhangang ^{[5
]}

Liu, Xiaodong ^{[2
]}

Choi, Gordon ^{[2
]}

Leung, Czarina C. H. ^{[2
]}

Wong, Wai T. ^{[2
]}

Li, Zheng ^{[6
,7
]}

Gin, Tony ^{[2
]}

Chan, Matthew T. V. ^{[2
]}

Wu, William K. K. ^{[1
,2
]}

机构：

[1] Chinese Univ Hong Kong, LKS Inst Hlth Sci, State Key Lab Digest Dis, Hong Kong, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Dept Anesthesia & Intens Care, Shatin, Hong Kong, Peoples R China

[3] Chinese Univ Hong Kong, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Jockey Club Sch Publ Hlth & Primary Care, Hong Kong, Hong Kong, Peoples R China

[5] Southwest Med Univ, Sch Pharm, Dept Pharmacol, Mol Pharmacol Lab, Luzhou 646000, Sichuan, Peoples R China

[6] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Orthoped Surg, Beijing, Peoples R China

[7] Peking Union Med Coll, Beijing, Peoples R China

来源：

CRITICAL CARE | 2016年 / 20卷

关键词：

Sepsis; microRNA; lncRNA; circRNA; Biomarker; Inflammation; Shock; NF-KAPPA-B; RESPIRATORY-DISTRESS-SYNDROME; INTERNATIONAL CONSENSUS DEFINITIONS; INFLAMMATORY RESPONSE SYNDROME; TNF-ALPHA PRODUCTION; ACUTE LUNG INJURY; MICRORNA EXPRESSION; GENE-EXPRESSION; DOWN-REGULATION; CARDIAC DYSFUNCTION;

D O I：

10.1186/s13054-016-1555-3

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Background: Sepsis coincides with altered gene expression in different tissues. Accumulating evidence has suggested that microRNAs, long non-coding RNAs, and circular RNAs are important molecules involved in the crosstalk with various pathways pertinent to innate immunity, mitochondrial functions, and apoptosis. Methods: We searched articles indexed in PubMed (MEDLINE), EMBASE and Europe PubMed Central databases using the Medical Subject Heading (MeSH) or Title/Abstract words ("microRNA", "long non-coding RNA", "circular RNA", "sepsis" and/or "septic shock") from inception to Sep 2016. Studies investigating the role of host-derived microRNA, long non-coding RNA, and circular RNA in the pathogenesis of and as biomarkers or therapeutics in sepsis were included. Data were extracted in terms of the role of non-coding RNAs in pathogenesis, and their applicability for use as biomarkers or therapeutics in sepsis. Two independent researchers assessed the quality of studies using a modified guideline from the Systematic Review Center for Laboratory animal Experimentation (SYRCLE), a tool based on the Cochrane Collaboration Risk of Bias tool. Results: Observational studies revealed dysregulation of non-coding RNAs in septic patients. Experimental studies confirmed their crosstalk with JNK/NF-kappa B and other cellular pathways pertinent to innate immunity, mitochondrial function, and apoptosis. Of the included studies, the SYRCLE scores ranged from 3 to 7 (average score of 4.55). This suggests a moderate risk of bias. Of the 10 articles investigating non-coding RNAs as biomarkers, none of them included a validation cohort. Selective reporting of sensitivity, specificity, and receiver operating curve was common. Conclusions: Although non-coding RNAs appear to be good candidates as biomarkers and therapeutics for sepsis, their differential expression across tissues complicated the process. Further investigation on organ-specific delivery of these regulatory molecules may be useful.

引用

页数：12

共 157 条

[91] microRNA-210 negatively regulates LPS-induced production of proinflammatory cytokines by targeting NF-κB1 in murine macrophages [J].