Circadian regulation of intracellular G-protein signalling mediates intercellular synchrony and rhythmicity in the suprachiasmatic nucleus

被引：106

作者：

Doi, Masao ^{[1
]}

Ishida, Atsushi ^{[2
]}

Miyake, Akiko ^{[2
]}

Sato, Miho ^{[1
]}

Komatsu, Rie ^{[1
]}

Yamazaki, Fumiyoshi ^{[1
]}

Kimura, Ikuo ^{[3
]}

Tsuchiya, Soken ^{[3
]}

Kori, Hiroshi ^{[4
,5
]}

Seo, Kazuyuki ^{[1
]}

Yamaguchi, Yoshiaki ^{[1
]}

Matsuo, Masahiro ^{[1
]}

Fustin, Jean-Michel ^{[1
]}

Tanaka, Rina ^{[1
]}

Santo, Yasuko ^{[1
]}

Yamada, Hiroyuki ^{[1
]}

Takahashi, Yukari ^{[1
]}

Araki, Michihiro ^{[6
]}

Nakao, Kazuki ^{[7
]}

Aizawa, Shinichi ^{[7
]}

Kobayashi, Masaki ^{[8
]}

Obrietan, Karl ^{[9
]}

Tsujimoto, Gozoh ^{[3
]}

Okamura, Hitoshi ^{[1
]}

机构：

[1] Kyoto Univ, Dept Syst Biol, Grad Sch Pharmaceut Sci, Kyoto 6068501, Japan

[2] Kobe Univ, Grad Sch Med, Div Mol Brain Sci, Kobe, Hyogo 6500017, Japan

[3] Kyoto Univ, Dept Genom Drug Discovery Sci, Grad Sch Pharmaceut Sci, Kyoto 6068501, Japan

[4] Ochanomizu Univ, Ochadai Acad Prod, Div Adv Sci, Tokyo 1128610, Japan

[5] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

[6] Kyoto Univ, Educ Unit Global Leaders, Kyoto 6068501, Japan

[7] Riken Ctr Dev Biol, Lab Anim Resources & Genet Engn, Kobe, Hyogo 6500047, Japan

[8] Tohoku Inst Technol, Dept Elect, Sendai, Miyagi 9828577, Japan

[9] Ohio State Univ, Dept Neurosci, Columbus, OH 43210 USA

来源：

NATURE COMMUNICATIONS | 2011年 / 2卷

关键词：

GENE-EXPRESSION; RESPONSE ELEMENT; CLOCK; MOUSE; TRANSCRIPTION; LIGHT; HYPOTHALAMUS; OSCILLATOR; INHIBITION; PROMOTERS;

D O I：

10.1038/ncomms1316

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Synchronous oscillations of thousands of cellular clocks in the suprachiasmatic nucleus (SCN), the circadian centre, are coordinated by precisely timed cell-cell communication, the principle of which is largely unknown. Here we show that the amount of RGS16 (regulator of G protein signalling 16), a protein known to inactivate Gai, increases at a selective circadian time to allow time-dependent activation of intracellular cyclic AMP signalling in the SCN. Gene ablation of Rgs16 leads to the loss of circadian production of cAMP and as a result lengthens circadian period of behavioural rhythm. The temporally precise regulation of the cAMP signal by clock-controlled RGS16 is needed for the dorsomedial SCN to maintain a normal phase-relationship to the ventrolateral SCN. Thus, RGS16-dependent temporal regulation of intracellular G protein signalling coordinates the intercellular synchrony of SCN pacemaker neurons and thereby defines the 24 h rhythm in behaviour.

引用

页数：9

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