Why does TNA cross-pair more strongly with RNA than with DNA? An answer from X-ray analysis

被引：59

作者：

Pallan, PS

Wilds, CJ

Wawrzak, Z

Krishnamurthy, R

Eschenmoser, A

Egli, M ^{[1
]}

机构：

[1] Vanderbilt Univ, Dept Biochem, Nashville, TN 37332 USA

[2] Concordia Univ, Dept Chem & Biochem, Montreal, PQ H4B 1R6, Canada

[3] Argonne Natl Lab, DND CAT Synchrotron Res Ctr, Argonne, IL 60439 USA

[4] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[5] ETH, Lab Organ Chem, CH-8093 Zurich, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 47期

关键词：

conformation analysis; DNA; RNA; structure elucidation; TNA;

D O I：

10.1002/anie.200352553

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The TNA twist: L-α-threofuranosyl (3′↔2′) nucleic acid (TNA) residues adopt a C4′-exo pucker when incorporated into an A-(left) or a B-form DNA duplex (right). The resulting intranucleotide P⋯P distance in TNA is very similar to that in RNA (represented by a C3′-endo puckered adenosine residue; green). The structural data explain earlier observations that TNA hydridizes more stably with RNA than with DNA and that RNA constitutes the better template for ligating TNA fragments.

引用

页码：5893 / 5895

页数：3