Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay

被引：83

作者：

Maksyutov, Rinat A. ^{[1
]}

Gavrilova, Elena V. ^{[1
]}

Shchelkunov, Sergei N. ^{[1
]}

机构：

[1] State Res Ctr Virol & Biotechnol VECTOR, Koltsov 630559, Novosibirsk Reg, Russia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2016年 / 236卷

关键词：

Variola virus; Monkeypox virus; Varicella-zoster virus; Real-time PCR; CONGO; REEMERGENCE; INFECTIONS; SMALLPOX; OUTBREAK;

D O I：

10.1016/j.jviromet.2016.07.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：215 / 220

页数：6

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