The use of green tea polyphenols for treating residual albuminuria in diabetic nephropathy: A double-blind randomised clinical trial

被引：89
作者：
Borges, Cynthia M. [1 ]
Papadimitriou, Alexandros [1 ]
Duarte, Diego A. [1 ]
de Faria, Jacqueline M. Lopes [1 ]
de Faria, Jose B. Lopes [1 ]
机构：
[1] State Univ Campinas UNICAMP, Fac Med Sci FCM, Renal Pathophysiol Lab, Invest Diabet Complicat,Nephrol Unit, Campinas, SP, Brazil
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
巴西圣保罗研究基金会;
关键词：
BLOOD-PRESSURE; GLUCOSE; INTERVENTION; PODOCYTES; THERAPY; PATHWAY; TARGET;
D O I：
10.1038/srep28282
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Prior research has shown that in experimental diabetes mellitus, green tea reduces albuminuria by decreasing podocyte apoptosis through activation of the WNT pathway. We investigated the effect of green tea polyphenols (GTP) on residual albuminuria of diabetic subjects with nephropathy. We conducted a randomised, double-blind study in 42 diabetic subjects with a urinary albumin-creatinine ratio (UACR) > 30 mg/g, despite administration of the maximum recommended dose of renin-angiotensin (RAS) inhibition. Patients were randomly assigned to two equal groups to receive either GTP (containing 800 mg of epigallocatechin gallate, 17 with type 2 diabetes and 4 with type 1 diabetes) or placebo (21 with type 2 diabetes) for 12 weeks. Treatment with GTP reduced UACR by 41%, while the placebo group saw a 2% increase in UACR (p = 0.019). Podocyte apoptosis (p = 0.001) and in vitro albumin permeability (p < 0.001) were higher in immortalized human podocytes exposed to plasma from diabetic subjects compared to podocytes treated with plasma from normal individuals. In conclusion, GTP administration reduces albuminuria in diabetic patients receiving the maximum recommended dose of RAS. Reduction in podocyte apoptosis by activation of the WNT pathway may have contributed to this effect.
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页数：9
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共 30 条
[1]   Novel mechanism of Wnt signalling inhibition mediated by Dickkopf-1 interaction with LRP6/Arrow [J].
Bafico, A ;
Liu, GZ ;
Yaniv, A ;
Gazit, A ;
Aaronson, SA .
NATURE CELL BIOLOGY, 2001, 3 (07) :683-686
[2]   Recognition, Pathogenesis, and Treatment of Different Stages of Nephropathy in Patients With Type 2 Diabetes Mellitus [J].
Bakris, George L. .
MAYO CLINIC PROCEEDINGS, 2011, 86 (05) :444-456
[3]   Effects of dosing condition on the oral bioavailability of green tea catechins after single-dose administration of Polyphenon E in healthy individuals [J].
Chow, HHS ;
Hakim, IA ;
Vining, DR ;
Crowel, JA ;
Ranger-Moore, J ;
Chew, WM ;
Celaya, CA ;
Rodney, SR ;
Hara, Y ;
Alberts, DS .
CLINICAL CANCER RESEARCH, 2005, 11 (12) :4627-4633
[4]   The renaissance of GSK3 [J].
Cohen, P ;
Frame, S .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2001, 2 (10) :769-776
[5]   Albuminuria, a therapeutic target for cardiovascular protection in type 2 diabetic patients with nephropathy [J].
de Zeeuw, D ;
Remuzzi, G ;
Parving, HH ;
Keane, WF ;
Zhang, ZX ;
Shahinfar, S ;
Snapinn, S ;
Cooper, ME ;
Mitch, WE ;
Brenner, BM .
CIRCULATION, 2004, 110 (08) :921-927
[6]   Proteinuria, a target for renoprotection in patients with type 2 diabetic nephropathy: Lessons from RENAAL [J].
de Zeeuw, D ;
Remuzzi, G ;
Parving, HH ;
Keane, WF ;
Zhang, ZX ;
Shahinfar, S ;
Snapinn, S ;
Cooper, MF ;
Mitch, WE ;
Brenner, BM .
KIDNEY INTERNATIONAL, 2004, 65 (06) :2309-2320
[7]   Autophagy Attenuates Diabetic Glomerular Damage through Protection of Hyperglycemia-Induced Podocyte Injury [J].
Fang, Li ;
Zhou, Yang ;
Cao, Hongdi ;
Wen, Ping ;
Jiang, Lei ;
He, Weichun ;
Dai, Chunsun ;
Yang, Junwei .
PLOS ONE, 2013, 8 (04)
[8]   Uncoupling Endothelial Nitric Oxide Synthase Is Ameliorated by Green Tea in Experimental Diabetes by Re-establishing Tetrahydrobiopterin Levels [J].
Faria, Aline M. ;
Papadirnitriou, Alexandros ;
Silva, Kamila C. ;
Lopes de Faria, Jacqueline M. ;
Lopes de Faria, Jose B. .
DIABETES, 2012, 61 (07) :1838-1847
[9]   EQUIVALENCE OF WEIGHTED KAPPA AND INTRACLASS CORRELATION COEFFICIENT AS MEASURES OF RELIABILITY [J].
FLEISS, JL ;
COHEN, J .
EDUCATIONAL AND PSYCHOLOGICAL MEASUREMENT, 1973, 33 (03) :613-619
[10]   Relationship of Dickkopf1 (DKK1) with Cardiovascular Disease and Bone Metabolism in Caucasian Type 2 Diabetes Mellitus [J].
Garcia-Martin, Antonia ;
Reyes-Garcia, Rebeca ;
Garcia-Fontana, Beatriz ;
Morales-Santana, Sonia ;
Coto-Montes, Ana ;
Munoz-Garach, Manuel ;
Rozas-Moreno, Pedro ;
Munoz-Torres, Manuel .
PLOS ONE, 2014, 9 (11)
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