Long-term histological and behavioural characterisation of a collagenase-induced model of intracerebral haemorrhage in rats

被引：22

作者：

Beray-Berthat, Virginie ^{[1
]}

Delifer, Celine ^{[1
]}

Besson, Valerie C. ^{[1
]}

Girgis, Haymen ^{[1
]}

Coqueran, Berard ^{[1
]}

Plotkine, Michel ^{[1
]}

Marchand-Leroux, Catherine ^{[1
]}

Margaill, Isabelle ^{[1
]}

机构：

[1] Univ Paris 05, Fac Sci Pharmaceut & Biol, Equipe Rech Pharmacol Circulat Cerebrale EA4475, F-75006 Paris, France

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2010年 / 191卷 / 02期

关键词：

Intracerebral haemorrhage; Collagenase; Behaviour; Sensorimotor tests; Beam-walking test; Neurological score; Adhesive removal test; Ipsilateral circling induced by dexamphetamine; ACTIVATED-RECEPTOR-GAMMA; MIDDLE CEREBRAL-ARTERY; SENSORIMOTOR RECOVERY; FUNCTIONAL RECOVERY; BRAIN-INJURY; STROKE; HEMATOMA; OUTCOMES; INFLAMMATION; ASYMMETRIES;

D O I：

10.1016/j.jneumeth.2010.06.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although intracerebral haemorrhage (ICH) entails the highest rates of mortality and disability of all stroke subtypes, efficient neuroprotective therapy is still needed. As functional recovery is a major endpoint in clinical trials, preclinical studies must demonstrate the potential of drugs to improve the sensorimotor and cognitive function of animals. In addition, behavioural studies should be performed on the long-term in order to truly mimic clinical needs. The aim of our study was to characterise a model of intracerebral haemorrhage using both histology and long-term behaviour. ICH was induced in rats by an intrastriatal injection of collagenase. Histology was performed 24 h, 7 days and 2 months after ICH. Among a set of sensorimotor tests, we discriminate those able to reveal long-term deficits (up to 2 months) after cerebral haemorrhage. Our five behavioural tests (a neurological score, an adhesive removal test, two beam-walking tests and ipsilateral circling induced by dexamphetamine) proved to be effective in revealing sensorimotor deficits up to 35 days or more after cerebral haemorrhage. In conclusion, these behavioural tests appear of particular interest to screen protective agents that may exhibit benefits in patients who suffer ICH. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：180 / 190

页数：11

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[1] Experimental animal models of intracerebral hemorrhage [J].