Leishmania species: Detection and identification by nested PCR assay from skin samples of rodent reservoirs

被引：72

作者：

Akhavan, Amir Ahmad ^{[1
]}

Mirhendi, Hossein ^{[2
]}

Khamesipour, Ali ^{[3
]}

Alimohammadian, Mohammad Hossein ^{[4
]}

Rassi, Yavar ^{[1
]}

Bates, Paul ^{[5
]}

Kamhawi, Shaden ^{[6
]}

Valenzuela, Jesus G. ^{[6
]}

Arandian, Mohammad Hossein ^{[7
]}

Abdoli, Hamid ^{[7
]}

Jalali-zand, Niloufar ^{[7
]}

Jafari, Reza ^{[7
]}

Shareghi, Niloufar ^{[7
]}

Ghanei, Maryam ^{[7
]}

Yaghoobi-Ershadi, Mohammad Reza ^{[1
]}

机构：

[1] Univ Tehran Med Sci, Sch Publ Hlth, Dept Med Entomol & Vector Control, Tehran, Iran

[2] Univ Tehran Med Sci, Sch Publ Hlth, Dept Med Parasitol & Mycol, Tehran, Iran

[3] Univ Tehran Med Sci, Ctr Res & Training Skin Dis & Leprosy, Tehran, Iran

[4] Pasteur Inst Iran, Dept Immunol, Tehran, Iran

[5] Univ Lancaster, Sch Hlth & Med, Div Biomed & Life Sci, Lancaster, England

[6] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD 20852 USA

[7] Univ Tehran Med Sci, Publ Hlth Res Inst, Esfahan Hlth Training & Res Ctr, Tehran, Iran

来源：

EXPERIMENTAL PARASITOLOGY | 2010年 / 126卷 / 04期

关键词：

Leishmania major; Leishmania gerbilli; Leishmania turanica; Rodent; Rhombomys opimus; Cutaneous leishmaniasis; Nested PCR; Iran; ZOONOTIC CUTANEOUS LEISHMANIASIS; RHOMBOMYS-OPIMUS; INFECTIONS; NUCLEAR; HOSTS; FOCI;

D O I：

10.1016/j.exppara.2010.06.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rodent skin samples. We designed Leishmania-specific primers that successfully amplified ITS regions of Leishmania major, Leishmania gerbilli and Leishmania turanica using nested PCR. Out of 95 field collected Rhombomys opimus, 21 were positive by microscopic examination and 48 by nested PCR. The percentage of gerbils infected with L. major, L gerbilli and L turanica was 3.2%, 1.1% and 27.4%, respectively. In 15.8% of the rodents, we found mixed natural infections by L. major and L turanica, 1.1% by L major and L gerbilli, and 2.1% by the three species. We concluded that this method is simple and reliable for detecting and identifying Leishmania species circulating in rodent populations. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：552 / 556

页数：5

共 24 条

[1]

Abdalla N. M., 2003, Infection Genetics and Evolution, V2, P277

[2]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[3] SPORADIC CUTANEOUS LEISHMANIASIS IN NORTH TUNISIA - IDENTIFICATION OF THE CAUSATIVE AGENT AS LEISHMANIA-INFANTUM BY THE USE OF A DIAGNOSTIC DEOXYRIBONUCLEIC-ACID PROBE [J].

BENISMAIL, R ;

SMITH, DF ;

READY, PD ;

AYADI, A ;

GRAMICCIA, M ;

BENOSMAN, A ;

BENRACHID, MS .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1992, 86 (05) :508-510

[4]

Dubrovsky YA, 1979, WHO TRAV SEM LEISHM

[5] A SIMPLE TECHNIQUE FOR PREPARATION OF SMEARS FROM THE EAR OF RHOMBOMYS-OPIMUS FOR THE DETECTION OF LEISHMANIAL INFECTION [J].

EDRISSIAN, GH ;

ZOVEIN, Z ;

NADIM, A .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1982, 76 (05) :706-707

[6]

Etemad E., 1978, RODENTS THEIR IDENTI, V1

[7]

EVANS D, 1989, UNDP WORLD BANK WHO

[8] Value of diagnostic techniques for cutaneous leishmaniasis [J].

Faber, WR ;

Oskam, L ;

van Gool, T ;

Kroon, NCM ;

Knegt-Junk, KJ ;

Hofwegen, H ;

van der Wal, AC ;

Kager, PA .

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 2003, 49 (01) :70-74

[9] The current status of zoonotic leishmaniases and approaches to disease control [J].

Gramiccia, M ;

Gradoni, L .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2005, 35 (11-12) :1169-1180

[10] Isolation and characterization of pathogenic Leishmania turanica from Nesokia indica (Rodentia, Muridae) by PCR-RFLP and ITS1 sequencing in Iran [J].

Hajjaran, H. ;

Mohebali, M. ;

Alimoradi, S. ;

Abaei, M. R. ;

Edrissian, Gh. H. .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2009, 103 (11) :1177-1179

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