Breaking the degeneracy of the genetic code

被引：102

作者：

Kwon, I ^{[1
]}

Kirshenbaum, K ^{[1
]}

Tirrell, DA ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 25期

关键词：

D O I：

10.1021/ja0350076

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons. Copyright © 2003 American Chemical Society.

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页码：7512 / 7513

页数：2

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