DNA Microcapsule for Photo-Triggered Drug Release Systems

被引：15
作者：
Kamiya, Yukiko [1 ,2 ]
Yamada, Yoshinobu [1 ]
Muro, Takahiro [1 ]
Matsuura, Kazunori [3 ]
Asanuma, Hiroyuki [1 ]
机构：
[1] Nagoya Univ, Dept Biomol Engn, Grad Sch Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
[2] Nagoya Univ, Inst Mat & Syst Sustainabil, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
[3] Tottori Univ, Dept Chem & Biotechnol, Grad Sch Engn, Koyama Minami 4-101, Tottori 6808552, Japan
来源：
CHEMMEDCHEM | 2017年 / 12卷 / 24期
关键词：
azobenzene; DNA; drug release; microcapsules; photo-trigger; SIRNA DELIVERY; CANCER-THERAPY; IN-VIVO; LIGHT; NANOPARTICLES; NANOMACHINE; CELLS; RNA; NANOSTRUCTURES; AZOBENZENES;
D O I：
10.1002/cmdc.201700512
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
In this study we constructed spherical photo-responsive microcapsules composed of three photo-switchable DNA strands. These strands first formed a three-way junction (TWJ) motif that further self-assembled to form microspheres through hybridization of the sticky-end regions of each branch. To serve as the photo-switch, multiple unmodified azobenzene (Azo) or 2,6-dimethyl-4-(methylthio)azobenzene (SDM-Azo) were introduced into the sticky-end regions via a d-threoninol linker. The DNA capsule structure deformed upon trans-to-cis isomerization of Azo or SDM-Azo induced by specific light irradiation. In addition, photo-triggered release of encapsulated small molecules from the DNA microcapsule was successfully achieved. Moreover, we demonstrated that photo-triggered release of doxorubicin caused cytotoxicity to cultured cells. This biocompatible photo-responsive microcapsule has potential application as a photo-controlled drug-release system.
引用
收藏
页码：2016 / 2021
页数：6
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