A prokaryotic acyl-CoA reductase performing reduction of fatty acyl-CoA to fatty alcohol

被引:80
作者
Hofvander, Per [1 ]
Doan, Thuy T. P. [1 ,2 ]
Hamberg, Mats [3 ]
机构
[1] Swedish Univ Agr Sci, Dept Plant Breeding & Biotechnol, SE-23053 Alnarp, Sweden
[2] Nong Lam Univ, Dept Biol, Ho Chi Minh City, Vietnam
[3] Karolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
关键词
Acyl-CoA reductase; Aldehyde reductase; Fatty alcohol; Fatty aldehyde; Marinobacter aquaeolei; MAMMALIAN WAX BIOSYNTHESIS; COENZYME-A SYNTHETASE; TRIACYLGLYCEROL BIOSYNTHESIS; POLLEN-WALL; ESTER; PURIFICATION; PROTEIN; IDENTIFICATION; ACCUMULATION; SYNTHASE;
D O I
10.1016/j.febslet.2011.10.016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The reduction of acyl-CoA or acyl-ACP to fatty alcohol occurs via a fatty aldehyde intermediate. In prokaryotes this reaction is thought to be performed by separate enzymes for each reduction step while in eukaryotes these reactions are performed by a single enzyme without the release of the intermediate fatty aldehyde. However, here we report that a purified fatty acyl reductase from Marinobacter aquaeolei VT8, evolutionarily related to the fatty acyl reductases in eukaryotes, catalysed both reduction steps. Thus, there are at least two pathways existing among prokaryotes for the reduction of activated acyl substrates to fatty alcohol. The Marinobacter fatty acyl reductase studied has a wide substrate range in comparison to what can be found among enzymes so far studied in eukaryotes. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:3538 / 3543
页数:6
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