Identification of Staphylococcus spp. by PCR-Restriction fragment length polymorphism of gap gene

被引：45
|
作者：
Yugueros, J
Temprano, A
Sánchez, M
Luengo, JM
Naharro, G [1 ]
机构：
[1] Univ Leon, Dept Sanidad Anim Microbiol & Immunol, E-24071 Leon, Spain
[2] Univ Leon, Dept Bioquim & Biol Mol, E-24071 Leon, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 10期
关键词：
D O I：
10.1128/JCM.39.10.3693-3695.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AIA digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.
引用
收藏
页码：3693 / 3695
页数：3
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